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Structure of the pro α2(I) collagen gene

Nature volume 294, pages 129–135 (1981)Cite this article

Abstract

Fifty-four kilobase pairs (kbp) of cloned chicken DNA containing the entire 38-kbp pro α2(I) collagen gene have been isolated and characterized. DNA sequence analysis of a select 4 kbp of the gene has precisely described 14 exons which comprise one-third of the sequences encoding the triple-helical domain of the collagen protein. These exons range in size from 45 to 108 base pairs (bp), are all multiples of the 9 bp that code for the repeating triplet, Gly-X-Y, and have an average size of 70 bp. About 50 introns interrupt this gene. Nevertheless, introns do not separate the coding sequences for the ends of the central triple-helical structural domain and the ends of the propeptide domains.

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Author notes

  1. John Wozney

    Present address: Center for Cancer Research, E17-336, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts, 02139, USA

Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Harvard University, 7 Divinity Avenue, Cambridge, Massachusetts, 02138, USA

    John Wozney, Douglas Hanahan, Valerie Tate, Helga Boedtker & Paul Doty

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  1. John Wozney
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  2. Douglas Hanahan
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  3. Valerie Tate
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  4. Helga Boedtker
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  5. Paul Doty
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Wozney, J., Hanahan, D., Tate, V. et al. Structure of the pro α2(I) collagen gene. Nature 294, 129–135 (1981). https://doi.org/10.1038/294129a0

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