Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter
  • Published:

Multiple copies of iso-insertion sequences of IS1 in Shigella dysenteriae chromosome

Nature volume 292, pages 640–643 (1981)Cite this article

Abstract

The insertion sequence, IS1, which is 768 base pairs(bp) long1, is the smallest active translocatable DNA element in bacteria (for review see ref. 2), and appears as a repeated DNA sequence in some Enterobacteriaceae species3. In particular, most Shigella species contain more than 40 copies of a sequence that hybridizes to IS13. Here, we report the isolation of various repeated sequences from the Shigella dysenteriae chromosome and the DNA sequence analysis of the class, which is abut 770 bp long. The results demonstrate that there are two major types of sequence in this class; one is identical to IS1 except for 10-bp substitutions and is present in about 50 copies in the chromosome; the second sequence, 766 bp long, is repeated more than 150 times and has ∼55% homology to IS1 on the nucleotide sequence level. Because of this high degree of homology, we call these ‘iso-insertion sequences’ of IS1. Further examination of the nucleotide sequence reveals common coding frames between these sequences, suggesting the existence of two genes that are required for multiplication of these sequences.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Ohtsubo, H. & Ohtsubo, E. Proc. natn. Acad. Sci. U.S.A. 75, 615–619 (1978).

    Article  ADS  CAS  Google Scholar 

  2. Calos, M. P. & Miller, J. H. Cell 20, 579–595 (1980).

    Article  CAS  Google Scholar 

  3. Nyman, K., Nakamura, K., Ohtsubo, H. & Ohtsubo, E. Nature 289, 609–612 (1981).

    Article  ADS  CAS  Google Scholar 

  4. Ohtsubo, H. & Ohtsubo, E. in DNA Insertion Elements, Plasmids and Episomes (eds Bukhari, A. I., Shapiro, J. A. & Adhya, S. L.) 49–63 (Cold Spring Harbor Laboratory, New York, 1977).

    Google Scholar 

  5. Brenner, D. J., Fanning, G. R., Skerman, F. J. & Falkow, S. J. Bact. 109, 953–965 (1972).

    CAS  PubMed  Google Scholar 

  6. Saito, H. & Miura, K. Biochim. biophys. Acta 72, 619–629 (1963).

    Article  CAS  Google Scholar 

  7. Maxam, A. M. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 74, 560–564 (1977).

    Article  ADS  CAS  Google Scholar 

  8. Johnsrud, L. Molec. gen. Genet. 169, 213–218 (1979).

    Article  CAS  Google Scholar 

  9. Calos, M. P., Johnsrud, L. & Miller, J. H. Cell 13, 411–418 (1978).

    Article  CAS  Google Scholar 

  10. Grindley, N. D. F. Cell 13, 419–426 (1978).

    Article  CAS  Google Scholar 

  11. Ohtsubo, E. et al. Cold Spring Harb. Symp. quant. Biol. 45, 283–295 (1981).

    Article  CAS  Google Scholar 

  12. Machida, Y., Machida, C., Ohtsubo, H. & Ohtsubo, E. Proc. natn. Acad. Sci. U.S.A. (in the press).

  13. Shine, J. & Dalgarno, L. Proc. natn. Acad. Sci. U.S.A. 71, 1342–1346 (1974).

    Article  ADS  CAS  Google Scholar 

  14. Gilbert, W. in RNA Polymerase (eds Losick, R. & Chamberlin, M.) 193–205 (Cold Spring Harbor Laboratory, New York, 1976).

    Google Scholar 

  15. Rosenberg, M. & Court, D. A. A. Rev. Genet. 13, 319–353 (1979).

    Article  CAS  Google Scholar 

  16. MacHattie, L. A. & Jackowski, J. in DNA Insertion Elements, Plasmids and Episomes (eds Bukhari, A. I., Shapiro, J. A. & Adhya, S. L.) 219–228 (Cold Spring Harbor Laboratory, New York, 1977).

    Google Scholar 

  17. So, M., Heffron, F. & McCarthy, B. Nature 277, 453–456 (1979).

    Article  ADS  CAS  Google Scholar 

  18. So, M. & McCarthy, B. Proc. natn. Acad. Sci. U.S.A. 77, 4011–4015 (1980).

    Article  ADS  CAS  Google Scholar 

  19. Johnsrud, L., Calos, M. P. & Miller, J. H. Cell 15, 1209–1219 (1978).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Microbiology, School of Medicine, State University of New York at Stony Brook, Stony Brook, New York, 11794, USA

    Hisako Ohtsubo, Kate Nyman, Wlodzimierz Doroszkiewicz & Eiichi Ohtsubo

Authors
  1. Hisako Ohtsubo
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kate Nyman
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Wlodzimierz Doroszkiewicz
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Eiichi Ohtsubo
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ohtsubo, H., Nyman, K., Doroszkiewicz, W. et al. Multiple copies of iso-insertion sequences of IS1 in Shigella dysenteriae chromosome. Nature 292, 640–643 (1981). https://doi.org/10.1038/292640a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/292640a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing