Abstract
Using a synthetic oligonucleotide primer, cloned DNA fragments, each containing the entire coding sequence of rat relaxin, have been isolated from a clone ‘bank’ of ovarian mRNA sequences. The nucleotide sequence of these clones demonstrates that relaxin is synthesized as a preprorelaxin molecule with an unexpectedly large connecting peptide of 105 amino acid residues.
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References
Belt, W. D., Anderson, L. L., Cavazos, L. F. & Melampy, R. M. Endocrinology 89, 1–10 (1971).
Anderson, L. L., Bast, J. D. & Melampy, R. M. J. Endocr. 59, 371–372 (1973).
O'Byrne, E. et al. J. clin. Endocr. Metab. 47, 1106–1110 (1978).
Schwabe, C. et al. Recent Prog. Horm. Res. 34, 123–211 (1978).
Porter, D. G. & Downing, S. J. Reprod. Fert. 52, 95–102 (1978).
James, R., Niall, H., Kwok, S. & Bryant-Greenwood, G. D. Nature 267, 544–546 (1977).
Niall, H. D. et al. in Proc. 15th Mid-West Conf. Endocr. Metab., 1980 (ed. Anderson, R.) (Plenum, New York, in the press).
Bradshaw, R. A. Rev. Biochem. 47, 191–216 (1978).
Tager, H. S. & Steiner, D. F. A. A. Rev. Biochem. 43, 509–538 (1974).
Gast, M. J., Mercado-Simmen, R., Niall, H. & Boime, I. Ann. N.Y.Acad. Sci. 343, 148–154 (1980).
John, M. J., Walsh, J. R., Borgesson, B. W. & Niall, H. D. Endocrinology 108, 726–729 (1981).
Ullrich, A. et al. Science 196, 1313–1319 (1977).
Seeburg, P. H., Shine, J., Martial, J. A., Baxter, J. D. & Goodman, H. M. Nature 270, 486–494 (1977).
Noyes, B. E., Mevarech, M., Stein, R. & Agarwal, K. L. Proc. nain. Acad. Sci. U.S.A. 76, 1770–1774 (1979).
Szostak, J. W. et al. Meth. Enzym. 68, 419–428 (1979).
Sherwood, O. D. & Crnekovic, V. E. Endocrinology 104, 893–897 (1979).
Bantle, J. A., Maxwell, I. H. & Kahn, W. E. Analyt. Biochem 72, 413–427 (1976).
Roychoudhury, R., Jay, E. & Wu, R. Nucleic Acids Res. 3, 863–877 (1976).
Chang, A. C. Y. et al. Nature 275, 617–624 (1978).
Grunstein, M. & Wallis, J. Meth. Enzym. 68, 379–389 (1979).
Richards, R. I., Shine, J., Ullrich, A., Wells, J. R. E. & Goodman, H. M. Nucleic Acids Res. 7, 1137–1146 (1979).
Proudfoot, N. J. & Brownlee, G. G. Nature 263, 211–214 (1976).
Hobart, P., Crawford, R., Shen, L., Pictet, R. & Rutter, W. J. Nature 288, 137–141 (1980).
Grantham, R., Gautier, C., Gouy, M., Mercier, R. & Pave, A. Nucleic Acids Res. 8, 49–62 (1980).
Steiner, D. F., Quinn, P. S., Chan, S. J., Marsh, J. & Tager, H. S. Ann. N.Y. Acad. Sci. 343, 1–16 (1980).
Rinderknecht, E. & Humbel, R. E. FEBS Lett. 89, 283–286 (1978).
Busse, W. D. & Carpenter, F. H. Biochemistry 15, 1649–1657 (1976).
Hamilton, J. W. et al. Proc. natn. Acad. Sci. U.S.A. 71, 653–656 (1974).
Maxam, A. M. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 74, 560–564 (1977).
Sanger, F., Nicklen, S. & Coulson, A. R. Proc. natn. Acad. Sci. U.S.A. 74, 5463–5467 (1977).
Walsh, J. R. & Niall, H. D. Endocrinology 107, 1258–1260 (1980).
Sherwood, O. D. Endocrinology 104, 886–892 (1979).
Kwok, S. C. M., Chamley, W. A. & Bryant-Greenwood, G. D. Biochem. biophys. Res. Commun. 82, 997–1005 (1978).
Nakanishi, S. et al. Nature 278, 423–434 (1979).
Lomedico, P. et al. Cell 18, 545–558 (1978).
Bell, G. I. et al. Nature 284, 26–32 (1980).
Perler, F. et al. Cell 20, 555–566 (1980).
Fiddes, J. C. et al. Proc. natn. Acad. Sci. U.S.A. 76, 4294–4298 (1979).
Wallis, M. Nature 284, 512 (1980).
Blundell, T. L. & Humbel, R. E. Nature 287, 781–787 (1980).
Anderson, M. L., Long, J. A. & Hayashida, T. Biol. Reprod. 13, 499–504 (1975).
Kendall, J. Z., Plopper, C. G. & Bryant-Greenwood, G. D. Biol. Reprod. 18, 94–98 (1974).
Hudson, P. et al. Endocrinology 108, 353–356 (1981).
Dubois, M. P. & Dacheux, J. L. Cell Tissue Res. 187, 201–214 (1978),
Bryant-Greenwood, G. D. in Proc. 15th Mid-West Conf. Endocr. Metab., 1980 (ed. Anderson, R.) (Plenum, New York, in the press).
Seeberg, P. H. et al. Cell 12, 157–165 (1977).
Denhardt, D. T. Biochem. biophys. Res. Commun. 23, 641–646 (1966).
Sanger, F. & Coulson, A. R. FEBS Lett. 87, 107–110 (1978).
Taylor, J. M., Illmersee, R. & Summers, J. Biochim. biophys. Acta 442, 324–330 (1976).
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Hudson, P., Haley, J., Cronk, M. et al. Molecular cloning and characterization of cDNA sequences coding for rat relaxin. Nature 291, 127–131 (1981). https://doi.org/10.1038/291127a0
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DOI: https://doi.org/10.1038/291127a0
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