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Molecular cloning of foot and mouth disease virus genome and nucleotide sequences in the structural protein genes

Nature volume 290, pages 800–802 (1981)Cite this article

Abstract

Foot and mouth disease virus (FMDV), of the family Picornaviridae, consists of a single-stranded RNA (∼8,000 nucleotides), the translation of which is initiated on the 3′ side of a 150-nucleotide poly(C) tract and yields a single polyprotein which is processed by host cell proteases into four primary products (Fig. 1)1–4. One or more virus-specified proteases further cleave these into the final products, the capsid proteins (VP1–4) being derived from the precursor p88 (for review see ref. 5). There are seven serotypes of the virus and as it has been shown that the immunizing activity of FMDV particles is associated primarily with VP1 (refs 6, 7), it seems likely that antigenic variation in FMDV is a result of changes in the structure of this protein. To further our understanding of this variation and as a first step in the possible development of FMDV vaccines from genetically manipulated microorganisms, we report here the construction and analysis of recombinant plasmids containing cDNA copies of the RNA. Comparison of the deduced amino acid sequence with the known polypeptide sequences shows that the NH2-termini of VP2 and VP3 are conserved between the A and O serotypes whereas that of VP1 (the immunizing antigen) varies by as much as 42 between serotypes.

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References

  1. Chatterjee, N. K., Bachrach, H. L. & Polatnick, J. J. Virol. 69, 369–377 (1976).

    Article  CAS  Google Scholar 

  2. Sangar, D. V., Rowlands, D. J., Harris, T. J. R. & Brown, F. Nature 268, 648–650 (1977).

    Article  ADS  CAS  Google Scholar 

  3. Rowlands, D. J., Harris, T. J. R. & Brown, F. J. Virol. 26, 335–343 (1978).

    CAS  PubMed  PubMed Central  Google Scholar 

  4. Sangar, D. V., Black, D. N., Rowlands, D. J., Harris, T. J. R. & Brown, F. J. Virol. 33, 59–68 (1980).

    CAS  PubMed  PubMed Central  Google Scholar 

  5. Sangar, D. V. J. gen. Virol. 45, 1–13 (1979).

    Article  CAS  Google Scholar 

  6. Wild, T. F. & Brown, F. J. gen. Virol. 1, 247–250 (1967).

    Article  CAS  Google Scholar 

  7. Wild, T. F., Burroughs, J. N. & Brown, F. J. gen. Virol. 4, 313–320 (1969).

    Article  CAS  Google Scholar 

  8. Emtage, J. S., Catlin, G. H. & Carey, N. H. Nucleic Acids Res. 6, 1221–1240 (1979).

    Article  CAS  Google Scholar 

  9. Hoeijmakers, J. H. J., Borst, P. Van den Burg, J., Weissmann, C. & Cross, G. A. M. Gene 8, 391–417 (1980).

    Article  CAS  Google Scholar 

  10. Twigg, A. J. & Sherratt, D. Nature 283, 216–218 (1980).

    Article  ADS  CAS  Google Scholar 

  11. Lederberg, J. & Cohen, S. N. J. Bact. 119, 1072–1074 (1974).

    CAS  Google Scholar 

  12. Boyer, H. W. & Roulland-Dussoix, D. J. molec. Biol. 41, 459–472 (1969).

    Article  CAS  Google Scholar 

  13. Eckhardt, T. Plasmid. 1, 584–588 (1978).

    Article  CAS  Google Scholar 

  14. Harris, T. J. R., Robson, K. J. H. & Brown, F. J. gen. Virol. 50, 403–418 (1980).

    Article  CAS  Google Scholar 

  15. Southern, E. M. J. molec. Biol. 98, 503–517 (1975).

    Article  CAS  Google Scholar 

  16. Hanahan, D. & Meselson, M. Gene 10, 63–67 (1980).

    Article  CAS  Google Scholar 

  17. Grunstein, M. & Hogness, D. S. Proc. natn. Acad. Sci. U.S.A. 72, 3961–3965 (1975).

    Article  ADS  CAS  Google Scholar 

  18. Birnboim, H. C. & Doty, J. Nucleic Acids Res. 7, 1513–1523 (1980).

    Article  Google Scholar 

  19. Porter, A. G., Fellner, P., Black, D. N., Rowlands, D. J., Harris, T. J. R. & Brown, F. Nature 276, 298–301 (1978).

    Article  ADS  CAS  Google Scholar 

  20. Harris, T. J. R. J. Virol. 36, 659–664 (1980).

    CAS  PubMed  PubMed Central  Google Scholar 

  21. Robson, K. J. H., Harris, T. J. R. & Brown, F. J. gen. Virol. 37, 271–276 (1977).

    Article  CAS  Google Scholar 

  22. Bachrach, H. L., Swaney, J. B. & Vande Woude, G. F. Virology 52, 520–528 (1973).

    Article  CAS  Google Scholar 

  23. Strohmaier, K., Wittman-Liebold, B. & Geissler, A. W. Biochem. biophys. Res. Commun. 85, 1640–1645 (1978).

    Article  CAS  Google Scholar 

  24. Bachrach, H. L., Morgan, D. O. & Moore, D. M. Intervirology 12, 65–72 (1979).

    Article  CAS  Google Scholar 

  25. Laporte, J., Grosclaude, J., Wantyghem, J., Serge, B. & Rouze, P. C. r. hebd. Acad. Sci. Séanc. Paris, 276, 3399–3401 (1973).

    CAS  Google Scholar 

  26. Meloen, R. H., Rowlands, D. J. & Brown, F. J. gen. Virol. 45, 761–763 (1979).

    Article  CAS  Google Scholar 

  27. Bachrach, H. L., Moore, D. M., McKercher, P. D. & Polatnick, J. J. Immun. 115, 1636–1641 (1975).

    CAS  PubMed  Google Scholar 

  28. Doel, T. R., Sangar, D. V., Rowlands, D. J. & Brown, F. J. gen. Virol. 41, 395–404 (1978).

    Article  CAS  Google Scholar 

  29. Küpper, H. et al. Nature 289, 555–559 (1981).

    Article  ADS  Google Scholar 

  30. Harris, T. J. R. Nucleic Acids Res. 7, 1765–1785 (1979).

    Article  CAS  Google Scholar 

  31. Maxam, A. M. & Gilbert, W. Meth. Enzym. 65, 499–560 (1980).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Immunochemistry, Wellcome Research Laboratories, Langley Court, Beckenham, Kent, BR3 3BS, UK

    John C. Boothroyd, Peter E. Highfield & George A. M. Cross

  2. Department of Biochemistry, Animal Virus Research Institute, Pirbright, Woking, Surrey, GU24 0NF, UK

    David J. Rowlands, Peter A. Lowe, Fred Brown & Timothy J. R. Harris

Authors
  1. John C. Boothroyd
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  2. Peter E. Highfield
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  6. Fred Brown
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  7. Timothy J. R. Harris
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Boothroyd, J., Highfield, P., Cross, G. et al. Molecular cloning of foot and mouth disease virus genome and nucleotide sequences in the structural protein genes. Nature 290, 800–802 (1981). https://doi.org/10.1038/290800a0

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