Abstract
Evidence points to the existence of multiple classes of dopamine (DA) receptor in mammalian brain which can be distinguished by their pharmacological specificities and localizations, and by the actions they mediate1. Among them, dopaminergic autoreceptors are regulatory receptors presumed to be present in the membrane of the DA neurones themselves, and believed to mediate an inhibition of these neurones' activity, either at nerve endings or on cell bodies2–5. However, the pharmacology of autoreceptors remains to be established because attempts to characterize autoreceptors by 3H-ligand binding techniques have produced controversial data. Thus Seeman and co-workers stated that 3H-apomorphine selectively labels autoreceptors6,7, whereas Creese et al. concluded that this ligand selectively labels postsynaptic DA receptors8,9. In addition, large differences in the capacity and drug specificity of 3H-apomorphine receptor sites in rat striatum have been reported10–13. We demonstrate here that 3H-apomorphine labels two classes of DA receptor, distinguishable using domperidone, a selective DA antagonist14–17. Lesion studies indicate that they correspond to a certain class of postsynaptic receptor and to autoreceptors, respectively. Each of these classes displays a clearly distinct pharmacological specificity for antipsychotics and DA agonists.
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References
Kebabian, J. W. & Calne, D. B. Nature 277, 93–96 (1979).
Carlsson, A. in Pre- and Post-synaptic Receptors, Annual ACNP Meet., Puerto-Rico (eds Usdin, E. & Bunney W. E.) (Dekker, New York, 1975).
Roth, R. H. Commun. Psychopharmac. 3, 429–445 (1979).
Aghajanian, G. K. & Bunney, B. S. Naunyn Schmiedeberg's Arch. Pharmacol. 297, 1–7 (1977).
Glowinski, J. & Cheramy, A. in Catecholamines: Basic and Clinical Frontier Vol. 1 (eds Usdin, E., Kopin, I. J. & Barchas, J.) 231–239 (Pergamon, New York, 1978).
Nagy, J. I., Lee, T., Seeman, P. & Fibiger, H. C. Nature 274, 278–281 (1978).
Weinreich, P., Lee, T. & Seeman, P. Soc. Neurosci. Abstr. 4, 1687 (1978).
Creese, I. & Snyder, S. H. Eur. J. Pharmac. 56, 277–281 (1979).
Creese, I., Usdin, T. & Snyder, S. H. Nature 278, 577–578 (1979).
Creese, I., Prosser, T. & Snyder, S. H. Life Sci. 23, 495–500 (1978).
Creese, I., Stewart, K. & Snyder, S. H. Eur. J. Pharmac. 60, 55–66 (1979).
Titeler, M., List, S. & Seeman, P. Commun. Psychopharmac. 3, 411–420 (1979).
Leysen, J. E. Commun. Psychopharmac. 3, 397–410 (1979).
Niemegeers, C. J. F. & Janssen, P. A. J. Proc. 7th int. Congr. Pharmac. Paris, Abstr. no. 68 (1978).
Martres, M. P., Baudry, M. & Schwartz, J. C. Life Sci. 23, 1781–1784 (1978).
Baudry, M., Martres, M. P. & Schwartz, J. C. Naunyn Schmiedeberg's Arch. Pharmac. 308, 231–237 (1979).
Watling, K. J., Dowling, J. E. & Iversen, L. L. Nature 281, 578–580 (1979).
Thal, L., Creese, I. & Snyder, S. H. Eur. J. Pharmac. 49, 295–299 (1978).
Sokoloff, P., Martres, M. P. & Schwartz, J.-C. Naunyn-Schmiedebergs Archs Pharmak. (in the press).
Creese, I., Burt, D. R. & Snyder, S. H. Science 197, 596–598 (1977).
Schwartz, J. C., Costentin, J., Martres, M. P., Pzotais, P. & Baudry, M. Neuropharmacology 17, 665–685 (1978).
Seeman, P., Chau-Wong, M., Tedesco, J. & Wong, K. Proc. natn. Acad. Sci. U.S.A. 72, 1376–1380 (1975).
Burt, D. R., Creese, I. & Snyder, S. H. Molec. Pharmac. 12, 800–812 (1976).
Leysen, J. E., Gommeren, W. & Laduron, P. M. Biochem. Pharmac. 27, 307–316 (1978).
Baudry, M., Martres, M. P. & Schwartz, J. C. in Catecholamines : Basic and Clinical Frontier Vol. 1 (eds Usdin, E., Kopin, I. J. & Barchas, J.) 565–567 (Pergamon, New York, 1978).
Martres, M. P., Sokoloff, P. & Schwartz, J.-C. in The Psychopharmacology and Bio-chemistry of Neurotransmitter Receptors (eds Olsen, R. N. & Yamamura, H. I.) (Elsevier, Amsterdam, in the press).
Trabucchi, M., Longoni, R., Fresia, P. & Spano, P. F. Life Sci. 17, 1551–1556 (1975).
Martres, M. P. et al. Brain Res. 136, 319–337 (1977).
Di Chiara, G., Porceddu, M. L., Fratta, W. & Gessa, G. L. Nature 267, 270–272 (1977).
Lieberman, A. N. et al. Adv. Neurol. 24, 461–473 (1979).
Tamminga, C. A., Schaffer, M. H., Smith, R. C. & Davis, J. M. in Catecholamines: Basic and Clinical Frontiers Vol. 2 (eds Usdin, E., Kopin, I. J. & Barchas, J.) 1836–1838 (Pergamon, New York, 1978).
Parker, R. B. & Waud, D. R. J. Pharmac. exp. Ther. 177, 1–12 (1971).
Albe-Fessard, D., Stutinsky, F. & Libouban, S. Editions du CNRS, Paris (1971).
Moskal, J. R. & Basu, S. Analyt. Biochem. 65, 449–457 (1975).
Aures, D., Håkanson, R. & Schauer, A. Eur. J. Pharmac. 3, 217–234 (1968).
König, F. R. & Klippel, R. A. The Rat Brain (Krieger, Huntington, New York, 1970).
Buda, M., Roussel, B., Renaud, B. & Pujol, J. F. Brain Res. 93, 564–569 (1975).
Llorens-Cortes, C., Pollard, H. & Schwartz, J. C. Neurosci. Lett. 12, 165–170 (1979).
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Sokoloff, P., Martres, MP. & Schwartz, JC. 3H-apomorphine labels both dopamine postsynaptic receptors and autoreceptors. Nature 288, 283–286 (1980). https://doi.org/10.1038/288283a0
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DOI: https://doi.org/10.1038/288283a0
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