Abstract
The sequences of two cloned rat pancreatic amylase cDNAs comprising 95% of the mRNA sequence are reported. Analysis of cloned rat genomic DNA fragments using cloned cDNA probes indicates that the rat genome contains multiple closely related amylase genes in which the cDNA sequences are distributed within a region 9 kilobases in length and are interrupted by at least seven intervening sequences.
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References
Sanders, T. G. thesis, Univ. Illinois (1970).
Loyter, A. & Schramm, M. Biochim. biophys. Acta 65, 200 (1962).
Arnold, M. & Rutter, W. J. J. biol. Chem. 238, 2760–2765 (1963).
Rutter, W. J., Wessells, N. K. & Grobstein, C. Natn. Cancer Inst. Monogr. 13, 51–65 (1964).
Kemp, J. D., Walther, B. T. & Rutter, W. J. J. biol. Chem. 247, 3941–3952 (1972).
Sanders, T. G. & Rutter, W. J. J. biol. Chem. 249, 3500–3509 (1974).
Van Nest, G. A., MacDonald, R. J., Raman, R. K. & Rutter, W. J. J. Cell Biol. (in the press).
Harding, J. D. et al. J. biol. Chem. 252, 7391–7397 (1977).
Przbyla, A. E., MacDonald, R. J., Harding, J. D., Pictet, R. L. & Rutter, W. J. J. biol. Chem. 254, 2154–2159 (1979).
Rutter, W. J., Ball, W. D., Bradshaw, W. S., Clark, W. R. & Sanders, T. G. in Secretory Mechanisms of Salivary Glands (eds Schneyer, L. H. & Schneyer, C. A.) 238–253 (Academic, New York, 1967).
Palla, J. C., Abdeljlil, A. B. & Desnuelle, P. Biochim. biophys. Acta 158, 25–35 (1968).
Rall, L. B., Pictet, R., Githens, S. & Rutter, W. J. J. Cell Biol. 75, 398–409 (1977).
Harding, J. D. & Rutter, W. J. J. biol. Chem. 253, 8736–8740 (1978).
Malacinski, G. M. & Rutter, W. J. Biochemistry 8, 4382–4390 (1969).
Sanders, T. G. & Rutter, W. J. Biochemistry 11, 130–136 (1972).
McGeachin, R. L. & Reynolds, J. M. J. biol. Chem. 234, 1456–1400 (1959); Biochim. biophys. Acta 39, 531–500 (1960).
MacDonald, R. J. et al. (in preparation).
Maxam, A. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 74, 560–564 (1977).
Bailey, J. M. & Davidson, N. Analyt. Biochem. 70, 75–85 (1976).
Lomedico, P. et al. Cell 18, 545–558 (1979).
Vandermeers, A. & Christophe, J. Biochim. biophys. Acta 154, 110–129 (1968).
Dayhoff, M. O., Hunt, L. T. & Hurst-Calderone, S. in Atlas of Protein Sequence and Structure Vol. 5, Suppl. 3 (ed. Dayhoff, M. O.) 363 (National Biomedical Research Foundation, Washington DC, 1978).
Ambler, R. P. Meth. Enzym. 25, 262–272 (1972).
Petra, D. H. Meth. Enzym. 19, 460–503 (1970).
Cozzone, P., Pasero, L., Beaupoil, B. & Marchis-Mouren, G. Biochim. biophys. Acta 207, 490–504 (1970).
Gorecki, M. & Zeelon, E. P. J. biol. Chem. 254, 525–529 (1979).
Blobel, G. & Dobberstein, B. J. Cell Biol. 67, 835–851 (1975).
Baralle, F. E. & Borwnlee, G. G. Nature 274, 84–87 (1978).
Nakanishi, S. et al. Nature 278, 423–427 (1979).
Ullrich, A. et al. Science 196, 1313–1319 (1977).
Nunberg, J. H., Kaufman, R. J., Chang, A. C. Y., Cohen, S. N. & Schimke, R. T. Cell 19, 355–364 (1980).
Hobart, P., Crawford, R., Shen, L.-P., Pictet, R. & Rutter, W. J. Nature (in the press).
Levitzki, A., Heller, J. & Schramm, M. Biochim. biophys. Acta 81, 101–107 (1964).
Loyter, A. & Schramm, M. J. biol. Chem. 241, 2611–2617 (1966).
Mora, S., Simon, I. & Elodi, P. Molec. Cell Biochem. 4, 205–209 (1974).
Robyt, J. F., Chittenden, C. G. & Lee, C. T. Archs. Biochem. Biophys. 144, 160–167 (1971).
Lehrner, L. N. & Malacinski, G. M. in Isozymes Vol. 4 (ed. Market, C. L.) 727–743 (Academic, New York, 1975).
Simon, I., Mora, S. & Elodi, P. Molec. Cell Biochem. 4, 211–216 (1974).
Fitzgerald, P. M. D., Stankiewicz, P. J., Smith, S. C. & McPherson, A. J. molec. Biol. 135, 753–756 (1979).
Payan, F., Haser, R., Pierrot, M., Frey, M. & Astier, J. P. Acta crystallogr. B36, 416–421 (1980).
McLachlan, A. D. J. molec. Biol. 61, 409–424 (1971).
Garnier, J., Osguthorpe, D. J. & Robson, B. J. molec. Biol. 120, 97–120 (1978).
Chou, P. Y. & Fasman, G. D. Biochemistry 13, 222–245 (1974).
Tang, J., James, M. N. G., Hsu, I. N., Jenkins, J. A. & Blundell, T. L. Nature 271, 618–721 (1978).
Ploegman, J. H., Drent, G., Kalk, K. H. & Hel, W. G. J. J. molec. Biol. 123, 557–594 (1978).
McLachlan, A. D. J. molec. Biol. 128, 49–79 (1979).
Sargent, T. D. et al. Proc. natn. Acad. Sci. U.S.A. 76, 3256–3260 (1979).
Benton, W. D. & Davis, R. W. Science 196, 180–182 (1977).
Breathnach, R., Benoist, C., O'Hare, K., Gannon, F. & Chambon, P. Proc. natn. Acad. Sci. U.S.A. 75, 4853–4857 (1978).
Dugaiczyk, A. et al. Nature 274, 328–333 (1978).
Cordell, B. et al. Cell 18, 533–543 (1979).
Bell, G. I. et al. Nature 284, 26–32 (1980).
Tsujimoto, Y. & Suzuki, Y. Cell 18, 591–600 (1979).
Goodman, H. M. & MacDonald, R. J. Meth. Enzym. 68, 75–79 (1979).
Fergusson, J. & Davis, R. W. in Advanced Techniques in Biological Electron Microscopy (ed. Koehler, J. K.) 123–171 (Springer, Berlin,1978).
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MacDonald, R., Crerar, M., Swain, W. et al. Structure of a family of rat amylase genes. Nature 287, 117–122 (1980). https://doi.org/10.1038/287117a0
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DOI: https://doi.org/10.1038/287117a0
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