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Structure of a family of rat amylase genes

Abstract

The sequences of two cloned rat pancreatic amylase cDNAs comprising 95% of the mRNA sequence are reported. Analysis of cloned rat genomic DNA fragments using cloned cDNA probes indicates that the rat genome contains multiple closely related amylase genes in which the cDNA sequences are distributed within a region 9 kilobases in length and are interrupted by at least seven intervening sequences.

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References

  1. Sanders, T. G. thesis, Univ. Illinois (1970).

  2. Loyter, A. & Schramm, M. Biochim. biophys. Acta 65, 200 (1962).

    Article  CAS  Google Scholar 

  3. Arnold, M. & Rutter, W. J. J. biol. Chem. 238, 2760–2765 (1963).

    CAS  PubMed  Google Scholar 

  4. Rutter, W. J., Wessells, N. K. & Grobstein, C. Natn. Cancer Inst. Monogr. 13, 51–65 (1964).

    CAS  Google Scholar 

  5. Kemp, J. D., Walther, B. T. & Rutter, W. J. J. biol. Chem. 247, 3941–3952 (1972).

    CAS  PubMed  Google Scholar 

  6. Sanders, T. G. & Rutter, W. J. J. biol. Chem. 249, 3500–3509 (1974).

    CAS  PubMed  Google Scholar 

  7. Van Nest, G. A., MacDonald, R. J., Raman, R. K. & Rutter, W. J. J. Cell Biol. (in the press).

  8. Harding, J. D. et al. J. biol. Chem. 252, 7391–7397 (1977).

    CAS  PubMed  Google Scholar 

  9. Przbyla, A. E., MacDonald, R. J., Harding, J. D., Pictet, R. L. & Rutter, W. J. J. biol. Chem. 254, 2154–2159 (1979).

    Google Scholar 

  10. Rutter, W. J., Ball, W. D., Bradshaw, W. S., Clark, W. R. & Sanders, T. G. in Secretory Mechanisms of Salivary Glands (eds Schneyer, L. H. & Schneyer, C. A.) 238–253 (Academic, New York, 1967).

    Google Scholar 

  11. Palla, J. C., Abdeljlil, A. B. & Desnuelle, P. Biochim. biophys. Acta 158, 25–35 (1968).

    Article  CAS  Google Scholar 

  12. Rall, L. B., Pictet, R., Githens, S. & Rutter, W. J. J. Cell Biol. 75, 398–409 (1977).

    Article  CAS  Google Scholar 

  13. Harding, J. D. & Rutter, W. J. J. biol. Chem. 253, 8736–8740 (1978).

    CAS  PubMed  Google Scholar 

  14. Malacinski, G. M. & Rutter, W. J. Biochemistry 8, 4382–4390 (1969).

    Article  CAS  Google Scholar 

  15. Sanders, T. G. & Rutter, W. J. Biochemistry 11, 130–136 (1972).

    Article  CAS  Google Scholar 

  16. McGeachin, R. L. & Reynolds, J. M. J. biol. Chem. 234, 1456–1400 (1959); Biochim. biophys. Acta 39, 531–500 (1960).

    Google Scholar 

  17. MacDonald, R. J. et al. (in preparation).

  18. Maxam, A. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 74, 560–564 (1977).

    Article  ADS  CAS  Google Scholar 

  19. Bailey, J. M. & Davidson, N. Analyt. Biochem. 70, 75–85 (1976).

    Article  CAS  Google Scholar 

  20. Lomedico, P. et al. Cell 18, 545–558 (1979).

    Article  CAS  Google Scholar 

  21. Vandermeers, A. & Christophe, J. Biochim. biophys. Acta 154, 110–129 (1968).

    Article  CAS  Google Scholar 

  22. Dayhoff, M. O., Hunt, L. T. & Hurst-Calderone, S. in Atlas of Protein Sequence and Structure Vol. 5, Suppl. 3 (ed. Dayhoff, M. O.) 363 (National Biomedical Research Foundation, Washington DC, 1978).

    Google Scholar 

  23. Ambler, R. P. Meth. Enzym. 25, 262–272 (1972).

    Article  CAS  Google Scholar 

  24. Petra, D. H. Meth. Enzym. 19, 460–503 (1970).

    Article  Google Scholar 

  25. Cozzone, P., Pasero, L., Beaupoil, B. & Marchis-Mouren, G. Biochim. biophys. Acta 207, 490–504 (1970).

    Article  CAS  Google Scholar 

  26. Gorecki, M. & Zeelon, E. P. J. biol. Chem. 254, 525–529 (1979).

    CAS  PubMed  Google Scholar 

  27. Blobel, G. & Dobberstein, B. J. Cell Biol. 67, 835–851 (1975).

    Article  CAS  Google Scholar 

  28. Baralle, F. E. & Borwnlee, G. G. Nature 274, 84–87 (1978).

    Article  ADS  CAS  Google Scholar 

  29. Nakanishi, S. et al. Nature 278, 423–427 (1979).

    Article  ADS  CAS  Google Scholar 

  30. Ullrich, A. et al. Science 196, 1313–1319 (1977).

    Article  ADS  CAS  Google Scholar 

  31. Nunberg, J. H., Kaufman, R. J., Chang, A. C. Y., Cohen, S. N. & Schimke, R. T. Cell 19, 355–364 (1980).

    Article  CAS  Google Scholar 

  32. Hobart, P., Crawford, R., Shen, L.-P., Pictet, R. & Rutter, W. J. Nature (in the press).

  33. Levitzki, A., Heller, J. & Schramm, M. Biochim. biophys. Acta 81, 101–107 (1964).

    CAS  Google Scholar 

  34. Loyter, A. & Schramm, M. J. biol. Chem. 241, 2611–2617 (1966).

    CAS  PubMed  Google Scholar 

  35. Mora, S., Simon, I. & Elodi, P. Molec. Cell Biochem. 4, 205–209 (1974).

    Article  CAS  Google Scholar 

  36. Robyt, J. F., Chittenden, C. G. & Lee, C. T. Archs. Biochem. Biophys. 144, 160–167 (1971).

    Article  CAS  Google Scholar 

  37. Lehrner, L. N. & Malacinski, G. M. in Isozymes Vol. 4 (ed. Market, C. L.) 727–743 (Academic, New York, 1975).

    Book  Google Scholar 

  38. Simon, I., Mora, S. & Elodi, P. Molec. Cell Biochem. 4, 211–216 (1974).

    Article  CAS  Google Scholar 

  39. Fitzgerald, P. M. D., Stankiewicz, P. J., Smith, S. C. & McPherson, A. J. molec. Biol. 135, 753–756 (1979).

    Article  CAS  Google Scholar 

  40. Payan, F., Haser, R., Pierrot, M., Frey, M. & Astier, J. P. Acta crystallogr. B36, 416–421 (1980).

    Article  Google Scholar 

  41. McLachlan, A. D. J. molec. Biol. 61, 409–424 (1971).

    Article  CAS  Google Scholar 

  42. Garnier, J., Osguthorpe, D. J. & Robson, B. J. molec. Biol. 120, 97–120 (1978).

    Article  CAS  Google Scholar 

  43. Chou, P. Y. & Fasman, G. D. Biochemistry 13, 222–245 (1974).

    Article  CAS  Google Scholar 

  44. Tang, J., James, M. N. G., Hsu, I. N., Jenkins, J. A. & Blundell, T. L. Nature 271, 618–721 (1978).

    Article  ADS  CAS  Google Scholar 

  45. Ploegman, J. H., Drent, G., Kalk, K. H. & Hel, W. G. J. J. molec. Biol. 123, 557–594 (1978).

    Article  CAS  Google Scholar 

  46. McLachlan, A. D. J. molec. Biol. 128, 49–79 (1979).

    Article  CAS  Google Scholar 

  47. Sargent, T. D. et al. Proc. natn. Acad. Sci. U.S.A. 76, 3256–3260 (1979).

    Article  ADS  CAS  Google Scholar 

  48. Benton, W. D. & Davis, R. W. Science 196, 180–182 (1977).

    Article  ADS  CAS  Google Scholar 

  49. Breathnach, R., Benoist, C., O'Hare, K., Gannon, F. & Chambon, P. Proc. natn. Acad. Sci. U.S.A. 75, 4853–4857 (1978).

    Article  ADS  CAS  Google Scholar 

  50. Dugaiczyk, A. et al. Nature 274, 328–333 (1978).

    Article  ADS  CAS  Google Scholar 

  51. Cordell, B. et al. Cell 18, 533–543 (1979).

    Article  CAS  Google Scholar 

  52. Bell, G. I. et al. Nature 284, 26–32 (1980).

    Article  ADS  CAS  Google Scholar 

  53. Tsujimoto, Y. & Suzuki, Y. Cell 18, 591–600 (1979).

    Article  CAS  Google Scholar 

  54. Goodman, H. M. & MacDonald, R. J. Meth. Enzym. 68, 75–79 (1979).

    Article  CAS  Google Scholar 

  55. Fergusson, J. & Davis, R. W. in Advanced Techniques in Biological Electron Microscopy (ed. Koehler, J. K.) 123–171 (Springer, Berlin,1978).

    Book  Google Scholar 

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MacDonald, R., Crerar, M., Swain, W. et al. Structure of a family of rat amylase genes. Nature 287, 117–122 (1980). https://doi.org/10.1038/287117a0

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