Abstract
The development of antigen-specific, T-cell lines has been a subject of intensive investigation by many laboratories interested in the problems of the nature of the T-cell receptor and the mechanism of major histocompatibility complex (MHC) restriction. We describe here a modification of the soft agar cloning technique which has allowed us to rapidly generate and expand proliferating T-lymphocyte clones directly from murine lymph nodes. Using this technique, we demonstrate that such T-cell clones are antigen specific and require I–A compatible antigen-presenting cells for stimulation.
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Sredni, B., Tse, H. & Schwartz, R. Direct cloning and extended culture of antigen-specific MHC-restricted, proliferating T lymphocytes. Nature 283, 581–583 (1980). https://doi.org/10.1038/283581a0
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DOI: https://doi.org/10.1038/283581a0
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