Abstract
The filamentous bacterial virus Pf1 is a simple model for biological filaments. We have studied the structure of the virion and report here that the helix parameters of Pf1 change sharply with temperature at about 8 °C. Local interactions between protein subunits change by only a few tenths of an ångstrom, but the changes are amplified between one end and the other of the virion to a rotation of 15 turns and a translation of 1,000 Å. The limited nature of the phase transition is probably due to the constraints of ‘knobs-into-holes’ interaction between side chains of adjacent α-helical protein subunits. Treatment of the virion with ether causes a rearrangement of protein subunits into sheets, with the α-helices normal to the plane of the sheet. This phase transition suggests a model for virion assembly in the bacterial membrane.
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Nave, C., Fowler, A., Malsey, S. et al. Macromolecular structural transitions in Pf1 filamentous bacterial virus. Nature 281, 232–234 (1979). https://doi.org/10.1038/281232a0
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DOI: https://doi.org/10.1038/281232a0
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