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Synthesis of rabbit β-globin in cultured monkey kidney cells following infection with a SV40 β-globin recombinant genome

Nature volume 277, pages 108–114 (1979)Cite this article

Abstract

Rabbit β-globin complementary DNA (cDNA) has been inserted into SV40DNA in place of the gene coding for the virus' major capsid protein, VP1. The recombinant genome, SVGT5-RaβG, multiplies efficiently in CV1 monkey kidney cell cultures and is transcribed to yield cytoplasmic, polyadenylated hybrid mRNAs containing the β-globin coding sequence. Cells propagating SVGT5-RaβG produce substantial quantities of rabbit β-globin polypeptide.

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Authors and Affiliations

  1. Department of Biochemistry, Stanford University Medical Center, Stanford, California, 94305

    Richard C. Mulligan, Bruce H. Howard & Paul Berg

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  1. Richard C. Mulligan
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  2. Bruce H. Howard
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  3. Paul Berg
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Mulligan, R., Howard, B. & Berg, P. Synthesis of rabbit β-globin in cultured monkey kidney cells following infection with a SV40 β-globin recombinant genome. Nature 277, 108–114 (1979). https://doi.org/10.1038/277108a0

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