Abstract
THE discovery of nitrogen-fixing activity associated with the rhizosphere of non-leguminous plants such as tropical grasses, rice and maize has provoked considerable interest1–9. Discrepancies in the measurements of this nitrogen-fixing activity by the acetylene reduction assay in different conditions prompted us to develop a modified assay system. We report here the use of this assay to find significant differences in the rates of acetylene reduction associated with the rhizosphere of 50 different strains of rice. This suggests that the rice plant genotype influences the association with nitrogen-fixing bacteria in its rhizosphere.
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References
Parker, C. A. Nature 179, 593–594 (1957).
Renaudo, G., Balandreau, P. & Dommergues, Y. Pl. Soil Spec. Vol.471–479 (1971).
Döbereiner, J., Day, J. M. & Dart, P. J. J. gen. Microbiol. 71, 103–116 (1972).
von Bülow, J. F. W. & Döbereiner, J. Proc. natn. Acad. Sci. U.S.A. 72, 2389–2393 (1975).
Yoshida, T. & Ancajas, R. R. Soil Sci. Pl. Nutr. 16, 234–237 (1970).
Balandreau, J., Miller, C. & Dommergues, Y. Appl. Microbiol. 27, 662–665 (1974).
Watanabe, I. Envir. Role Nitrogen-Fixing Blue-green Algae Asymbiotic Bacteria, Int. Symp., Uppsala (1976).
Lee, K. K., Castro, T. & Yoshida, T. Pl. Soil 48, 613–619 (1977).
Dommergues, Y., Balandreau, J., Rinaudo, G. & Weinhard, P. Soil Biol. Biochem. 5, 83–89 (1973).
Burris, R. H., Okon, Y. & Albrecht, S. Envir. Role Nitrogen Fixing Blue-green Algae Asymbiotic Bacteria, Int. Symp., Uppsala (1976).
Watanabe, I. et al. IRRI Res. Pap. Ser. 3, 1–16 (1977).
Yoshida, T. & Ancajas, R. R. Soil Sci. Am. Proc. 37, 42–46 (1973).
Lee, K. K. & Yoshida, T. Pl. Soil 46, 127–134 (1977).
Okon, Y., Albrecht, S. L. & Burris, R. H. Appl. envir. Microbiol. 33, 85–88 (1977).
Burris, R. H., Okon, Y. & Albrecht, S. L. Genetic Engineering for Nitrogen Fixation (ed. Hollaender, A.) 445–450 (Plenum, New York, 1977).
Hardy, R. W. F., Holsten, R. D., Jackson, E. K. & Burns, R. C. Pl. Physiol. 43, 1185–1207 (1968).
Burris, R. H. Meth. Enzym. 24, 415–431 (1972).
Döbereiner, J., Day, J. M. & Dart, P. J. Pl. Soil 37, 191–196 (1972).
Willemin, G., Balandreau, J. & Dommergues, Y. Ann. Microbiol. 24, 87–94 (1974).
Balandreau, J. et al. Limitations and Potentials for Biological Nitrogen Fixation in the Tropics (eds Döbereiner, J. et al. )275–302 (Plenum, New York, 1978).
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HIROTA, Y., FUJII, T., SANO, Y. et al. Nitrogen fixation in the rhizosphere of rice. Nature 276, 416–417 (1978). https://doi.org/10.1038/276416a0
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DOI: https://doi.org/10.1038/276416a0
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