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Synthesis of an ovalbumin-like protein by Escherichia coli K12 harbouring a recombinant plasmid

Abstract

A cloned DNA transcript of ovalbumin mRNA was cut a few nucleotides away from the initiator codon, and fused in phase to the beginning of the Escherichia coli β-galactosidase gene. The hybrid gene has been cloned in E. coli where it produces large amounts of an ovalbumin-like protein.

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References

  1. Struhl, K., Cameron, J. R. & Davis, R. W. Proc. natn. Acad. Sci. U.S.A. 73, 1471–1475 (1977).

    Article  ADS  Google Scholar 

  2. Struhl, K. & Davis, R. W. Proc. natn. Acad. Sci. U.S.A. 74, 5255–5259 (1977).

    Article  ADS  CAS  Google Scholar 

  3. Ratzkin, B. & Carbon, J. Proc. natn. Acad. Sci. U.S.A. 74, 487–491 (1977).

    Article  ADS  CAS  Google Scholar 

  4. Clarke, L. & Carbon, J. J. molec. Biol. 120, 517–532 (1978).

    Article  CAS  PubMed  Google Scholar 

  5. Vapnek, D., Hautala, J., Jacobson, J., Giles, N. & Kushner, S. Proc. natn. Acad. Sci. U.S.A. 74, 3058–3062 (1977).

    Article  Google Scholar 

  6. Rambach, A. & Hogness, D. S. Proc. natn. Acad. Sci. U.S.A. 74, 5041–5045 (1977).

    Article  ADS  CAS  Google Scholar 

  7. Polisky, B., Bishop, R. J. & Gelfand, D. M. Proc. natn. Acad. Sci. U.S.A. 73, 3900–3904 (1976).

    Article  ADS  CAS  Google Scholar 

  8. Kourilsky, P., Gros, D., Rougeon, F. & Mach, B. Nature 267, 637–639 (1977).

    Article  ADS  CAS  PubMed  Google Scholar 

  9. Itakura, K. et al. Science 198, 1056–1063 (1977).

    Article  ADS  CAS  PubMed  Google Scholar 

  10. Jeffreys, A. J. & Flavell, R. A. Cell 12, 1097–1108 (1977).

    Article  CAS  PubMed  Google Scholar 

  11. Tilghman, S. M. et al. Proc. natn. Acad. Sci. U.S.A. 75, 725–729 (1978).

    Article  ADS  CAS  Google Scholar 

  12. Breathnach, R., Mandel, J. L. & Chambon, P. Nature 270, 314–319 (1977).

    Article  ADS  CAS  PubMed  Google Scholar 

  13. Brack, C. & Tonegawa, S. Proc. natn. Acad. Sci. U.S.A. 74, 5652–5656 (1977).

    Article  ADS  CAS  Google Scholar 

  14. Goodman, H. M., Olson, M. V. & Hall, B. D. Proc. natn. Acad. Sci. U.S.A. 74, 5453–5457 (1977).

    Article  ADS  CAS  Google Scholar 

  15. Valenzuela, P. et al. Proc. natn. Acad. Sci. U.S.A. 75, 190–194 (1978).

    Article  ADS  CAS  Google Scholar 

  16. Garapin, A. C. et al. Nature 273, 349–358 (1978).

    Article  ADS  CAS  PubMed  Google Scholar 

  17. Garapin, A. C. et al. Cell (in the press).

  18. Mandel, J. L. et al. Cell (in the press).

  19. Doel, M. T., Houghton, M., Cook, E. A. & Carey, N. H. Nucleic Acids Res. 4, 3701–3713 (1977).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. Weinstock, R. et al. Proc. natn. Acad. Sci. U.S.A. 75, 1299–1303 (1978).

    Article  ADS  CAS  Google Scholar 

  21. Fothergill, L. A. & Fothergill, J. E. Biochem. J. 116, 555–561 (1970).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  22. Palmiter, R. O., Gagnon, J. & Walsh, A. Proc. natn. Acad. Sci. U.S.A. 75, 94–98 (1978).

    Article  ADS  CAS  Google Scholar 

  23. Lee, U. C. & Montgomery, R. Archs Biochem. Biophys. 97, 9–17 (1962).

    Article  CAS  Google Scholar 

  24. Milstein, C. P. Biochem. J. 110, 127–134 (1968).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  25. Wiseman, R. L., Fothergill, J. E. & Fothergill, L. A. Biochem. J. 127, 775–780 (1972).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  26. McReynolds, L. A. et al. Nature 273, 723 (1978).

    Article  ADS  CAS  PubMed  Google Scholar 

  27. McReynolds, L. A., Catterall, J. F. & O'Malley, B. W. Gene 2, 217–231 (1977).

    Article  CAS  Google Scholar 

  28. Humphries, P. et al. Nucleic Acids Res. 4, 2389–2407 (1977).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  29. Rougeon, F., Kourilsky, P. & Mach, B. Nucleic Acids Res. 2, 2365–2378 (1975).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  30. Rougeon, F. & Mach, B. J. biol. Chem. 252, 2209–2217 (1977).

    CAS  PubMed  Google Scholar 

  31. Breathnach, R., Benoist, C., O'Hara, K., Gannon, F. X. & Chambon, P., in preparation.

  32. Backman, K., Ptashne, M. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 73, 4174–4178 (1976).

    Article  ADS  CAS  Google Scholar 

  33. Charnay, P., Louise, A., Perrin, D. & Tiollais, P. Molec. gen. Genet. (submitted).

  34. Bolivar, F. et al. Gene 1, 65–79 (1977).

    Google Scholar 

  35. Cami, B. & Kourilsky, P. Nucleic Acids Res. (in the press).

  36. Fiers, W. et al. Nature 260, 500–507 (1976).

    Article  ADS  CAS  PubMed  Google Scholar 

  37. Sanger, F. Nature 265, 687–695 (1977).

    Article  ADS  CAS  PubMed  Google Scholar 

  38. Prog. scient. 191, 91–94 (1977).

  39. Dickson, R. C., Abelson, J., Barnes, W. M. & Reznikoff, W. S. Science 187, 27–35 (1975).

    Article  ADS  CAS  PubMed  Google Scholar 

  40. Sharp, P. et al. Biochemistry 12, 3053–3063 (1977).

    Google Scholar 

  41. Brown, N. L. & Smith, M. FEES Lett. 65, 284–287 (1976).

    Article  CAS  Google Scholar 

  42. Smith, D. L., Blattner, F. R. & Davies, J. Nucleic Acids Res. 3, 343–353 (1976).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  43. Perricaudet, M. & Tiollais, P. FEBS Lett. 56, 7–11 (1975).

    Article  CAS  PubMed  Google Scholar 

  44. Haggerty, D. M. & Schleif, R. E. J. Virol. 18, 659–663 (1976).

    CAS  PubMed  PubMed Central  Google Scholar 

  45. Bolton, A. E. & Hunter, W. M. Biochem. J. 133, 529–539 (1973).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  46. Stephens, J. C., Artz, S. W. & Ames, B. N. Proc. natn. Acad. Sci. U.S.A. 72, 4389–4393 (1975).

    Article  ADS  CAS  Google Scholar 

  47. Rhoads, R. E., McKnight, G. S. & Schimke, R. T. J. biol. Chem. 248, 2031–2039 (1973).

    CAS  PubMed  Google Scholar 

  48. Marsden, H. S., Crombie, I. K. & Subak-Sharpe, J. H. J. gen. Virol 31, 347–372 (1976).

    Article  CAS  PubMed  Google Scholar 

  49. Studier, F. W. J. molec. Biol. 79, 237–248 (1973).

    Article  CAS  PubMed  Google Scholar 

  50. Laskey, R. A. & Mills, A. D. Eur. J. Biochem. 56, 335–341 (1975).

    Article  CAS  PubMed  Google Scholar 

  51. Ullrich, A. et al. Science 196, 1313–1320 (1977).

    Article  ADS  CAS  PubMed  Google Scholar 

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Mercereau-Puijalon, O., Royal, A., Cami, B. et al. Synthesis of an ovalbumin-like protein by Escherichia coli K12 harbouring a recombinant plasmid. Nature 275, 505–510 (1978). https://doi.org/10.1038/275505a0

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