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Cellular transformation and the ‘morphologic phenotype’ of transformed cells

Nature volume 274, pages 645–650 (1978)Cite this article

Abstract

Expression of the product of the transforming gene (src) of RNA tumour viruses promotes growth and usually alters the adhesion, appearance and surface properties of cultured fibroblasts. The latter group of properties termed the ‘morphologic phenotype’ of transformed cells is largely due to diminished cell-to-substratum adhesion. The role of cyclic AMP, cell surface protein (CSP) and other factors in producing the ‘morphologic phenotype’ are discussed. The effects of src expression bear a striking resemblance to the action of peptide hormones such as insulin on appropriate target cells.

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References

  1. Pastan, I. et al. in International Cell Biology 1976–1977 (eds Brinkley, B. R. & Porter, K. R.) 161–167 (1977).

    Google Scholar 

  2. Yamada, K. M. & Weston, J. A. Proc. natn. Acad. Sci. U.S.A. 71, 3492–3496 (1974).

    Article  ADS  CAS  Google Scholar 

  3. Keski-Oja, J., Mosher, D. F. & Vaheri, A. Biochem. biophys. Res. Commun. 74, 699–706 (1977).

    Article  CAS  PubMed  Google Scholar 

  4. Hynes, R. O. Biochim. biophys. Acta 458, 73–107 (1976).

    CAS  PubMed  Google Scholar 

  5. Johnson, G. S., Friedman, R. M. & Pastan, I. Proc. natn. Acad. Sci. U.S.A. 68, 425–429 (1971).

    Article  ADS  CAS  Google Scholar 

  6. Hsie, A. W. & Puck, T. T. Proc. natn. Acad. Sci. U.S.A. 68, 358–361 (1971).

    Article  ADS  CAS  Google Scholar 

  7. Willingham, M. C. Int. Rev. Cytol. 44, 319–363 (1976).

    Article  CAS  PubMed  Google Scholar 

  8. Johnson, G. S. & Pastan, I. Nature new Biol. 237, 267–268 (1972).

    Article  CAS  PubMed  Google Scholar 

  9. Sheppard, J. R. Proc. natn. Acad. Sci. U.S.A. 68, 1316–1320 (1971).

    Article  ADS  CAS  Google Scholar 

  10. Hsie, A. W., Jones, C. & Puck, T. T. Proc. natn. Acad. Sci. U.S.A. 68, 1648–1652 (1971).

    Article  ADS  CAS  Google Scholar 

  11. Willingham, M. C. & Pastan, I. J. Cell Biol. 63, 288–294 (1974).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Willingham, M. & Pastan, I. Proc. natn. Acad. Sci. U.S.A. 72, 1263–1267 (1975).

    Article  ADS  CAS  Google Scholar 

  13. Ryan, W. L. & Heidrick, M. L. Science 162, 1484–1485 (1968).

    Article  ADS  CAS  PubMed  Google Scholar 

  14. Burk, R. R. Nature 219, 1272–1275 (1968).

    Article  ADS  Google Scholar 

  15. Johnson, G. S. & Pastan, I. J. natn. Cancer Inst. 48, 1377–1387 (1972).

    CAS  Google Scholar 

  16. Willingham, M. & Pastan, I. J. Cell Biol. 67, 146–159 (1975).

    Article  CAS  PubMed  Google Scholar 

  17. Otten, J., Johnson, G. & Pastan, I. Biochem. biophys. Res. Commun. 44, 1192–1198 (1971).

    Article  CAS  PubMed  Google Scholar 

  18. Carchman, R. A., Johnson, G. S., Pastan, I. & Scolnick, E. M. Cell 1, 59–64 (1974).

    Article  CAS  Google Scholar 

  19. Anderson, W. B., Russell, T. R., Carchman, R. A. & Pastan, I. Proc. natn. Acad. Sci. U.S.A. 70, 3802–3805 (1973).

    Article  ADS  CAS  Google Scholar 

  20. Burstin, S. J., Renger, H. C. & Basilico, C. J. cell. Physiol. 84, 69–74 (1974).

    Article  CAS  PubMed  Google Scholar 

  21. Oey, J., Vogel, A. & Pollack, R. Proc. natn. Acad. Sci. U.S.A. 71, 694–698 (1974).

    Article  ADS  CAS  Google Scholar 

  22. Pastan, I. H., Johnson, G. S. & Anderson, W. B. A. Rev. Biochem. 44, 491–522 (1975).

    Article  CAS  Google Scholar 

  23. Anderson, W. B., Gallo, M. & Pastan, I. J. biol. Chem. 249, 7041–7048 (1974).

    CAS  PubMed  Google Scholar 

  24. Pastan, I. & Johnson, G. S. Adv. Cancer Res. 19, 303–329 (1974).

    Article  CAS  PubMed  Google Scholar 

  25. Somers, K. D., Rachmeler, M. & Christensen, M. Nature 257, 58–60 (1975).

    Article  ADS  CAS  PubMed  Google Scholar 

  26. Somers, K. D., Weberg, A. D. & Steiner, S. J. Cell Biol. 74, 707–716 (1977).

    Article  CAS  PubMed  Google Scholar 

  27. DeLarco, J. & Todaro, G. J. J. cell. Physiol. 94, 335–342 (1978).

    Article  CAS  Google Scholar 

  28. Oler, A., Iannaccone, P. M. & Gordon, G. B. In Vitro 9, 35–38 (1973).

    Article  CAS  PubMed  Google Scholar 

  29. De Asua, L. J., Surian, E. S., Flavia, M. M. & Torres, H. N. Proc. natn. Acad. Sci. U.S.A. 70, 1388–1392 (1973).

    Article  ADS  Google Scholar 

  30. Willingham, M., Carchman, R. & Pastan, I. Proc. natn. Acad. Sci. U.S.A. 70, 2906–2910 (1973).

    Article  ADS  CAS  Google Scholar 

  31. Hochman, J., Insel, P. A., Bourne, H. R., Coffino, P. & Tomkins, G. M. Proc. natn. Acad. Sci. U.S.A. 72, 5051–5055 (1975).

    Article  ADS  CAS  Google Scholar 

  32. Davies, P., Shizuta, Y., Olden, K., Gallo, M. & Pastan, I. Biochem. biophys. Res. Commun. 74, 300–307 (1977).

    Article  CAS  PubMed  Google Scholar 

  33. Klein, I., Willingham, M. & Pastan, I. Expl. Cell Res. (in the press).

  34. Wang, K., Ash, J. F. & Singer, S. J. Proc. natn. Acad. Sci. U.S.A. 72, 4483–4486 (1975).

    Article  ADS  CAS  Google Scholar 

  35. Sherline, P. & Shiavone, K. J. Cell Biol. 77, R9–R12 (1978).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  36. Olden, K. & Yamada, K. M. Cell 11, 957–969 (1977).

    Article  CAS  PubMed  Google Scholar 

  37. Adams, S. L., Sobel, M. E., Howard, B. H., Olden, K., Yamada, K., de Crombrugghe, B. & Pastan, I. Proc. natn. Acad. Sci. U.S.A. 74, 3399–3404 (1977).

    Article  ADS  CAS  Google Scholar 

  38. Yamada, K., Yamada, S. & Pastan, I. Proc. natn. Acad. Sci. U.S.A. 73, 1217–1221 (1976).

    Article  ADS  CAS  Google Scholar 

  39. Yamada, K. M., Ohanian, S. H. & Pastan, I. Cell 9, 241–245 (1976).

    Article  CAS  PubMed  Google Scholar 

  40. Yamada, K. M. & Pastan, I. J. cell. Physiol. 89, 827–830 (1976).

    Article  CAS  PubMed  Google Scholar 

  41. Teng, N. N. H. & Chen, L. B. Proc. natn. Acad. Sci. U.S.A. 72, 413–417 (1975).

    Article  ADS  CAS  Google Scholar 

  42. Willingham, M. C., Yamada, K. M., Yamada, S. S., Pouyssegur, J. & Pastan, I. Cell 10, 375–380 (1977).

    Article  CAS  PubMed  Google Scholar 

  43. Pouyssegur, J. & Pastan, I. Proc. natn. Acad. Sci. U.S.A. 73, 544–548 (1976).

    Article  ADS  CAS  Google Scholar 

  44. Pouyssegur, J., Willingham, M. & Pastan, I. Proc. natn. Acad. Sci. U.S.A. 74, 243–247 (1977).

    Article  ADS  CAS  Google Scholar 

  45. Neufeld, E. J. & Pastan, I. Archs Biochem. Biophys. (in the press).

  46. Aub, J. C., Sanford, B. H. & Cote, M. D. Proc. natn. Acad. Sci. U.S.A. 54, 396–399 (1965).

    Article  ADS  CAS  Google Scholar 

  47. Burger, M. M. Proc. natn. Acad. Sci. U.S.A. 62, 994–1001 (1969).

    Article  ADS  CAS  Google Scholar 

  48. Inbar, B. & Sachs, L. Proc. natn. Acad. Sci. U.S.A. 63, 1418–1425 (1969).

    Article  ADS  CAS  Google Scholar 

  49. Nicolson, G. L. Nature new Biol. 243, 218–220 (1973).

    Article  CAS  PubMed  Google Scholar 

  50. Kosower, N. S., Kosower, E. M., Lustig, S. & Pluznik, D. H. Biochim. biophys. Acta 507, 128–136 (1978).

    Article  CAS  PubMed  Google Scholar 

  51. Hatten, M. E., Scandella, C. J., Horintz, A. F. & Burger, M. M. J. biol. Chem. 253, 1972–1977 (1978).

    CAS  PubMed  Google Scholar 

  52. Ukena, T. E. & Karnovsky, M. J. Expl. Cell Res. 106, 309–325 (1977).

    Article  CAS  Google Scholar 

  53. Collard, J. G. & Temmink, J. H. M. J. Cell Biol. 68, 101–112 (1976).

    Article  CAS  PubMed  Google Scholar 

  54. Ciba Fdn Symp. 14 (1973).

  55. Tooze, J. The Molecular Biology of Tumour Viruses (Cold Spring Harbour Monograph, 1973).

    Google Scholar 

  56. Bader, J. P. J. Virol. 10, 267–276 (1972).

    CAS  PubMed  PubMed Central  Google Scholar 

  57. Beng, H., Peters, J. H. & Graf, T. Z. Naturforsch. 31, 766–768 (1976).

    Article  Google Scholar 

  58. Anderson, W. B., Johnson, G. S. & Pastan, I. Proc. natn. Acad. Sci. U.S.A. 70, 1055–1059 (1973).

    Article  ADS  CAS  Google Scholar 

  59. Anderson, W. B., Lovelace, E. & Pastan, I. Biochem. biophys. Res. Commun. 52, 1293–1299 (1973).

    Article  CAS  PubMed  Google Scholar 

  60. Yoshida, M., Ikawa, Y., Owada, M., Toyoshima, K. Int. J. Cancer 20, 560–563 (1977).

    Article  CAS  PubMed  Google Scholar 

  61. Otten, J., Bader, J., Johnson, G. S. & Pastan, I. J. biol. Chem. 247, 1632–1633 (1972).

    CAS  PubMed  Google Scholar 

  62. Hori, T., Kesi-Oja, J. & Vaheri, A. Cell 2, 235–240 (1974).

    Article  Google Scholar 

  63. Kawai, S. & Hanafusa, H. Virology 46, 470–479 (1971).

    Article  CAS  PubMed  Google Scholar 

  64. Martin, G. S., Venuta, S., Weber, M. & Rubin, H. Proc. natn. Acad. Sci. U.S.A. 68, 2739–2741 (1971).

    Article  ADS  CAS  Google Scholar 

  65. Berg, H. & Graf, J. Cold Springs Harb. Symp quant. Biol. (in the press).

  66. Levinson, W., Bhatrayar, R. S. & Liu, T. Z. J. natn. Cancer Inst. 55, 807–810 (1975).

    Article  CAS  Google Scholar 

  67. Kamine, J. & Rubin, H. J. cell. Physiol. 92, 1–12 (1977).

    Article  CAS  PubMed  Google Scholar 

  68. Hata, R. & Peterfosky, B. Proc. natn. Acad. Sci. U.S.A. 74, 2933–2937 (1977).

    Article  ADS  CAS  Google Scholar 

  69. Jay, G., Shiu, R. P. C., Jay, F. T., Levine, A. S. & Pastan, I. Cell 13, 527–534 (1978).

    Article  CAS  PubMed  Google Scholar 

  70. Brugge, J. S. & Erikson, R. L. Nature 269, 346–348 (1977).

    Article  ADS  CAS  PubMed  Google Scholar 

  71. Kamine, J., Burr, J. G. & Buchanan, J. M. Proc. natn. Acad. Sci. U.S.A. 75, 366–370 (1978).

    Article  ADS  CAS  Google Scholar 

  72. Pastan, I., Roth, J. & Macchia, V. Proc. natn. Acad. Sci. U.S.A. 56, 1802–1809 (1966).

    Article  ADS  CAS  Google Scholar 

  73. Kahn, R. J. Cell Biol. 70, 261–286 (1976).

    Article  CAS  PubMed  Google Scholar 

  74. Fain, J. N. in Biochemistry Series One, MTP International Review of Science (Rickenberg, H. V. ed.) 8, 1–23 (1974).

    Google Scholar 

  75. Evans, R. B., Morhenn, V., Jones, A. L. & Tomkhis, G. M. J. Cell Biol. 61, 95–106 (1974).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  76. Mackall, J. C., Student, A. K., Polakis, S. E. & Lane, M. D. J. biol. Chem. 251, 6462–6464 (1976).

    CAS  PubMed  Google Scholar 

  77. Kuri-Haruch, W. & Green, H. J. biol. Chem. 252, 2158–2160 (1977).

    Google Scholar 

  78. Hutchings, S. E. & Sata, G. H. Proc. natn. Acad. Sci. U.S.A. 75, 901–904 (1978).

    Article  ADS  CAS  Google Scholar 

  79. Shiu, R., Pouyssegur, J. & Pastan, I. Proc. natn. Acad. Sci. U.S.A. 74, 3840–3844 (1977).

    Article  ADS  CAS  Google Scholar 

  80. Robison, G. A., Butcher, R. W. & Sutherland, E. W. Cyclic AMP (Academic, New York, 1971).

    Google Scholar 

  81. Schlessinger, J., Shechter, Y., Willingham, M. C. & Pastan, I. Proc. natn. Acad. Sci. U.S.A. (in the press).

  82. Goldfine, I. D., Smith, G. J., Wong, K. Y. & Jones, A. L. Proc. natn. Acad. Sci. U.S.A. 74, 1368–1372 (1977).

    Article  ADS  CAS  Google Scholar 

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  1. National Cancer Institute, Bethesda, Maryland, 20014

    Ira Pastan & Mark Willingham

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  1. Ira Pastan
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Pastan, I., Willingham, M. Cellular transformation and the ‘morphologic phenotype’ of transformed cells. Nature 274, 645–650 (1978). https://doi.org/10.1038/274645a0

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