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Intracellular binding sites for insulin are immunologically distinct from those on the plasma membrane

Abstract

SPECIFIC high affinity binding sites for insulin have been shown to be located on the plasma membrane of target cells1, and they are also present on purified intracellular organelles2 including intact nuclei3, smooth and rough endoplasmic reticulum2,4, and the Golgi apparatus5. Although these intracellular sites have certain binding characteristics similar to those on the plasma membrane2–5, it is not known whether these binding sites share other biochemical features. Using an antiserum directed at the cell surface binding sites, we have found that several types of intracellular binding sites for insulin are immunologically distinct from those on the plasma membrane.

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GOLDFINE, I., VIGNERI, R., COHEN, D. et al. Intracellular binding sites for insulin are immunologically distinct from those on the plasma membrane. Nature 269, 698–700 (1977). https://doi.org/10.1038/269698a0

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