Abstract
Evidence is presented that a small protein (VPg) is covalently attached to the 5′-terminal oligonucleotide VPg–pU-U–A–A–A–A–C–A–Gp of the polio genome, to nascent strands of the polio replicative intermediate and to poly(U) of minus strands. A model of polio RNA replication is proposed implicating VPg in initiation of RNA synthesis, possibly as primer.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Fiers, W., in RNA Phages (ed. Zinder, N. D.) 353–396 (Cold Spring Harbor, 1975).
Shatkin, A. J. Cell 9, 645–653 (1976).
Wimmer, E., Chang, A. Y., Clark, J. M. & Reichmann, E. M. J. molec. Biol. 38, 59–73 (1968).
Wimmer, E. J. molec. Biol. 68, 537–540 (1972).
Nomoto, A., Lee, Y. F. & Wimmer, E. Proc. natn. Acad. Sci. U.S.A. 73, 375–380, (1976).
Hewlett, M. J., Rose, J. K. & Baltimore, D. Proc. natn. Acad. Sci. U.S.A. 73, 327–330 (1976).
Fernandez-Muñoz, R. & Darnell, J. E. J. Virol. 18, 719–726 (1976).
Frisby, D., Eaton, M. & Fellner, P. Nucleic Acids Res. 3, 2771–2781 (1976).
Fernandez-Muñoz, R. & Lavi, U. J. Virol. 21, 820–824 (1977).
Lee, Y. F., Nomoto, A. & Wimmer, E. in Prog. Nucleic Acid Res. molec. Biol. 19, 89–96 (1976).
Lee, Y. F., Nomoto, A., Detjen, B. M. & Wimmer, E. Proc. natn. Acad. Sci. U.S.A. 74, 59–63 (1977).
Fujimoto, M., Kuninaka, A. & Yoshino, H. Agr. Biol. Chem. 38, 1555–1561 (1974).
Tomlinson, R. V. & Tener, G. M. Biochemistry 2, 703–706 (1963).
Lee, Y. F. & Wimmer, E. Nucleic Acids Res. 3, 1647–1658 (1976).
Uchida, T., Arima, T. & Egami, F. J. Biochem. (Tokyo) 67, 91–102 (1970).
Newman, J. F. E., Rowlands, D. J. & Brown, F. J. gen. Virol. 18, 171–180 (1973).
Baltimore, D., in Biochemistry of Viruses (ed. Levy, H. B.) 103–176 (Marcel Dekker, New York and London, 1969).
Yogo, Y. & Wimmer, E. J. molec. Biol. 92, 467–477 (1975).
Yogo, Y. & Wimmer, E. Nature new Biol. 242, 171–174 (1973).
Yogo, Y., Teng, M.-H. & Wimmer, E. Biochem. biophys. Res. Commun. 61, 1101–1109 (1974).
Rueckert, R. R. Compreh. Virol. 6, 131–213 (1976).
Dorsch-Häesler, K., Yogo, Y. & Wimmer, E. J. Virol. 16, 1512–1527 (1975).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Nomoto, A., Detjen, B., Pozzatti, R. et al. The location of the polio genome protein in viral RNAs and its implication for RNA synthesis. Nature 268, 208–213 (1977). https://doi.org/10.1038/268208a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/268208a0
This article is cited by
-
Regulation of RIG-I-like receptor-mediated signaling: interaction between host and viral factors
Cellular & Molecular Immunology (2021)
-
Variable 3’polyadenylation of Wheat yellow mosaic virus and its novel effects on translation and replication
Virology Journal (2019)
-
MicroRNA-134 regulates poliovirus replication by IRES targeting
Scientific Reports (2017)
-
Ribose 2′-O-methylation provides a molecular signature for the distinction of self and non-self mRNA dependent on the RNA sensor Mda5
Nature Immunology (2011)
-
Protein-primed RNA synthesis by purified poliovirus RNA polymerase
Nature (1998)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
