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Precise location of the crossover region in the λ attachment sequence

Nature volume 267, pages 555–557 (1977)Cite this article

Abstract

A PREREQUISITE for studying the DNA-protein interactions involved in integration and excision of λ is the determination of the DNA sequences of the attachment sites. Useful sequence data can only be obtained if the position of the attachment site is known precisely. To focus on this site we have used genetically- and physically-defined deletions and substitutions ending in att as reference points whose position relative to the sites of action of restriction endonucleases can be determined. In this way we have located the middle of the attachment site to within ± 10 base pairs in a molecule 46,500 base pairs long1.

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Author notes

  1. R. WAYNE DAVIES

    Present address: Department of Biology, University of Essex, Wivenhoe Park, Colchester, CO4 3SQ, UK

  2. T. G. FANNING

    Present address: Department of Biological Chemistry, Medical School, University of California, Davis, California, 95616

Authors and Affiliations

  1. Institut für Genetik der Universität zu Köln, Weyertal 121, D-5000, Köln, 41, FRG

    P. H. SCHREIER, R. WAYNE DAVIES, D. E. BÜCHELL, B. GRONENBORN, T. G. FANNING & B. VON WILCKEN

  2. Max Planck Institut für Biochemie, 8033, Martinsreid, München, FRG

    J. MESSING

Authors
  1. P. H. SCHREIER
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  2. R. WAYNE DAVIES
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  3. D. E. BÜCHELL
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  4. B. GRONENBORN
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  5. T. G. FANNING
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  6. B. VON WILCKEN
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  7. J. MESSING
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SCHREIER, P., DAVIES, R., BÜCHELL, D. et al. Precise location of the crossover region in the λ attachment sequence. Nature 267, 555–557 (1977). https://doi.org/10.1038/267555a0

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