Abstract
RELIABLE quantitative data on spontaneous, specific gene mutation frequencies in higher plants and animals are few. Detergents include low natural frequencies and difficulty in obtaining in excess of 106 scorable organisms or gametophytes. The alcohol dehydrogenase-1 gene (Adh1 gene; ADH enzyme EC 1.1.1.1.), as expressed in pollen grains, is among the exceptionably suitable; much is known about the maize ADHs1–3, Adh1 function is totally dispensible in an aerobic environment and maize pollen is a trinucleate gametophyte which expresses much of its haploid genome, including Adh14,5. In particular, there have been two recent methodological advances. First, I am able to cytochemically stain pollen, before5 or after in vitro germination, for the presence of above 5% normal ADH activity. And second, ADH1− pollen grains survive allyl alcohol (C=C–C–OH) vapour concentrations which kill ADH1+ grains6,7; this selection scheme was developed for yeast by Megnet8. My genetic resolution is approximately one mutant (Adh1+→ADH−) per 107 chemically selected, viable gametophytes, and one (phenotypic) revertant (Adh1−→ADH+) per 108 unselected gatetophytes. In this note, I compare spontaneous forward mutant frequency with previously published5 revertant frequencies for one naturally occurring and six ethyl methanesulphonate-induced Adhl-deficient (Adh1−) alleles.
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References
Schwartz, D. Proc. natn. Acad. Sci. U.S.A. 56, 1431–1436 (1966).
Felder, M. R., Scandalios, J. G. & Liu, E. H. Biochim. biophys. Acta 318, 149–159 (1973).
Freeling, M. & Schwartz, D. Biochem. Genet. 8, 27–36 (1973).
Schwartz, D. Genetics 67, 411–425 (1971).
Freeling, M. Genetics 83, 701–717 (1976).
Schwartz, D. & Osterman, J. Genetics 83, 63–65 (1976).
Cheng, D. S. K. & Freeling, M. Maize Genet. Coop. News Lett. 50, 11–13 (1976).
Megnet, R. Archs Biochem. Biophys. 121, 194–201 (1967).
Judd, B. H., Shen, M. W. & Kaufman, T. C. Genetics 71, 139–156 (1972).
Abrahamson, S., Bender, M. A., Conger, A. D. & Wolff, S. Nature 245, 460–461 (1973).
Cook, F. & Walden, D. Can. J. Bot. 43, 779–786 (1965).
Drake, J. W. J. cell comp. Physiol. 64, Suppl. 1, 19–32 (1964).
Freese, E. Proc. natn. Acad. Sci. U.S.A. 45, 623–633 (1959).
Brockman, H. E. & de Serres, F. J. Genetics 48, 597–604 (1963).
De Serres, F. J. Genetics 43, 187–206 (1958).
Stadler, L. J. The Spragg Memorial Lectures (Third Series) 3–15 (Michigan State College, East Lansing, 1942).
Nelson, O. E. Genetics 60, 607–524 (1968).
Schlager, G. & Dickie, M. M. Genetics 57, 319–330 (1967).
Auerbach, C. & Kilbey, B. J. A. Rev. Genet. 5, 163–218 (1971).
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FREELING, M. Spontaneous forward mutation versus reversion frequencies for maize Adh1 in pollen. Nature 267, 154–156 (1977). https://doi.org/10.1038/267154a0
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DOI: https://doi.org/10.1038/267154a0
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