Abstract
PEPTIDE hormone receptors in target tissues have been extensively analysed in vitro by binding studies with radio-active hormone derivatives. Although these methods have been of great value for characterisation of the binding sites for peptide hormones, there is also need for methods to examine the localisation and distribution of hormone receptors in isolated target cells. For this purpose, we have used an immunofluorescence technique to label the luteinising hormone (LH) receptors of the interstitial cells of the rat testis. This method has demonstrated that LH receptors are distributed as patches on the cell surface, and are rapidly lost from about 50% of the labelled cells after hypophysectomy. Also, occupancy of receptors by endogenous LH was found to persist for several days in a significant proportion of the interstitial cells.
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References
DeKretser, D. M., Catt, K. J., Burger, H. G., and Smith, G. C., J. Endocr., 43, 105–111 (1969).
Castro, A. E., Alonso, A., and Mancini, R. E., J. Endocr., 52, 129–136 (1972).
Catt, K. J., Dufau, M. L., and Tsuruhara, T., J. Clin. Endocr. Metab., 34, 123–132 (1972).
Catt, K. J., Tsuruhara, T., and Dufau, M. L., Biochim. biophys. Acta, 279, 194–201 (1972).
Catt, K. J., and Dufau, M. L., Adv. exp. Med. Biol., 36, 379–418 (1973).
Mendelson, C., Dufau, M. L., and Catt, K. J., J. biol. Chem., 250, 8818–8823 (1975).
Catt, K. J., Tsuruhara, T., Mendelson, C., Ketelslegers, J.-M., and Dufau, M. L., in Current Topics in Molecular Endocrinology, 1 (edit. by Dufau, M. L., and Means, A. R.), 1–34 (Plenum, New York, 1974).
Dufau, M. L., Mendelson, C., and Catt, K. J., J. clin. Endocr. Metab., 39, 610–613 (1974).
Frowein, J., and Engel, W., J. Endocr., 64, 59–66 (1975).
Ketelslegers, J.-M., Knott, G. D., and Catt, K. J., Biochemistry, 14, 3075–3083 (1975).
Dufau, M. L., Charreau, E. H., and Catt, K. J., J. biol. Chem., 248, 6973–6981 (1973).
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HSUEH, A., DUFAU, M., KATZ, S. et al. Immunofluorescence labelling of gonadotropin receptors in enzyme-dispersed interstitial cells. Nature 261, 710–712 (1976). https://doi.org/10.1038/261710a0
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DOI: https://doi.org/10.1038/261710a0
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