Abstract
The DNA of avian sarcoma virus assumes a closed circular configuration before integration into the host cell chromosomal DNA. Ethidium bromide reduces the formation of superhelical viral DNA and concurrently blocks integration of the viral genome. Inhibition of integration of viral DNA results in the inhibition of virus replication.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Temin, H. M., A. Rev. Microbiol., 25, 609–648 (1971).
Varmus, H. E., Vogt, P. K., and Bishop, J. M., Proc. natn. Acad, Sci. U.S.A., 70, 3067–3071 (1973).
Varmus, H. E., Vogt, P. K., and Bishop, J. M., J. molec. Biol., 74, 613–626 (1973).
Fridlender, B., and Weissbach, A., Proc. natn. Acad. Sci. U.S.A., 68, 3116–3119 (1971).
Guntaka, R. V., Bishop, J. M., and Varmus, H. E., Fundamental Aspects of Neoplasia (edit. by Gottlieb, A.) (Springer, in the press).
Varmus, H. E., Guntaka, R. V., Deng, C. T., and Bishop, J. M., Cold Spring Harb. Symp. quant. Biol., 39 (in the press).
Hirt, B., J. molec. Biol., 26, 365–369 (1967).
Radloff, R., Bauer, W., and Vinograd, J., Proc. natn. Acad. Sci. U.S.A., 57, 1514–1521 (1967).
Garapin, A. C., Varmus, H. E., Faras, A. J., Levinson, W. E., and Bishop, J. M., Virology, 52, 264–274 (1973).
Parsons, J. T., Coffin, J. M., Haroz, R. K., Bromley, P. A., and Weissman, C., J. Virol., 11, 761–774 (1973).
Reichert, N. J., and Hare, J. D., Biochem. Biophys. Res. Commun., 45, 5–10, (1972).
Bader, A., J. Virol., 11, 314–324 (1973).
Gazzolo, L. de-The, G., Vigier, P., and Sarma, P., C. r. hebd. Séanc. Acad. Sci., Paris, 268, 1668–1670 (1969).
Mach, O., and Kara, J., Folia Biol., 17, 65–72 (1971).
Penman, S., Fan, H., Perlman, S., Rosbach, M., Weinberg, R., and Zylber, E., Cold Spring Harb. Symp. quant. Biol., 35, 561–575 (1970).
Varmus, H. E., Guntaka, R. V., Fan, W., Heasley, S., and Bishop, J. M., Proc. natn. Acad. Sci. U.S.A., 71, 3874–3878 (1974).
Gelb, L. D., Kohne, D. E., and Martin, M. A., J. molec. Biol., 57, 129–145 (1971).
Leong, J. A., Garapin, A. C., Jackson, N., Fanshier, L., Levinson, W., and Bishop, J. M., J. Virol., 9, 891–902 (1972).
Tooze, J. (ed.), The Molecular Biology of Tumour Viruses (Cold Spring Harbor Laboratory, New York, 1973).
Varmus, H. E., Guntaka, R. V., Dominik, C., Deng, C. T., and Bishop, J. M., Prov. ninth Int. Cancer Cong. (in the press).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Guntaka, R., Mahy, B., Bishop, J. et al. Ethidium bromide inhibits appearance of closed circular viral DNA and integration of virus-specific DNA in duck cells infected by avian sarcoma virus. Nature 253, 507–511 (1975). https://doi.org/10.1038/253507a0
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1038/253507a0
This article is cited by
-
Development and characterization of continuous avian cell lines depleted of mitochondrial DNA
In Vitro Cellular & Developmental Biology (1988)
-
Covalently closed circles of adenovirus 5 DNA
Nature (1983)
-
Viral-specific antigen synthesis following de novo RD-114 virus infection of stationary cells
Current Microbiology (1979)
-
Effect of dibutyryl cyclic AMP on intracellular levels of avian sarcoma virus specific RNA
Nature (1978)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.