Abstract
THE usual pattern of chromosome segregation in interspecific humanĂ—mouse somatic cell hybrids is retention of mouse and loss of human chromosomes1. In attempts to generate hybrid strains expressing differentiated functions, we have fused an actively dividing human cell line with freshly dissociated, highly differentiated cells from the mouse embryonic nervous system. The majority of the resulting hybrid strains retain human while losing mouse chromosomes and isozymes and thus provide a new class of hybrid cell for analysis of gene linkage and regulation.
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References
Weiss, M., and Green, H., Proc. natn. Acad. Sci. U.S.A., 58, 1104–1111 (1967).
Weiss, M., Ephrussi, B., and Scaletta, L., Proc. natn. Acad. Sci. U.S.A., 59, 1132–1135 (1968).
Littlefield, J. W., Science, 145, 709–710 (1974).
Coon, H. G., Horak, I., Dawid, I. B., J. molec. Biol., 81, 285–298 (1973).
Coon, H. G., and Weiss, M. C., Proc. natn. Acad. Sci. U.S.A., 62, 852–859 (1969).
Minna, J., Glazer, D., and Nirenberg, M., Nature new Biol., 235, 225–231 (1972).
Lowry, O., Rosebrough, N. G., Farr, A. L., and Randall, R. J., J. biol. Chem., 193, 265–275 (1951).
Brewer, G. J., An introduction to isozyme techniques (Academic Press, New York, 1970).
Lewis, W. H. P., and Truslove, G. M., Biochem. Genet., 3, 493–498 (1969).
Edwards, Y. H., Hopkinson, D. A., and Harris, H., Ann. Human Genetics, 34, 395 (1971).
Nichols, E. A., and Ruddle, F. H., In Vitro, 7, 120–131 (1971).
Omenn, G. S., and Cohen, P. T. W., In Vitro, 7, 132–139 (1971).
Ruddle, F., Nature, 242, 165–169 (1973).
Nabholz, M., Miggiano, V., and Bodmer, W., Nature, 223, 358–363 (1969).
Shows, T., Proc. natn. Acad. Sci. U.S.A., 69, 348–352 (1972).
Norum, R. A., and Migeon, B. R., Nature, 251, 72–74 (1974).
Epstein, C. J., Science, 175, 1467–1468 (1972).
Jami, J. S., Grandchamp, S., and Ephrussi, B., C.r. hebd. Séanc. Acad. Sci., 272, 323–327 (1971); Jami, J. and Grandchamp, S., Proc. natn. Acad. Sci. U.S.A., 68, 3097–3101 (1971).
Miller, O. J., in Chromosome identification (edit. by Caspersson, T., and Zech, L.), 177 (Academic Press, New York, 1973).
Minna, J. D., Gazdar, A. F., Iverson, G. M., Marshall, T. H., Stromberg, K., and Wilson, S. W., Proc. natn. Acad. Sci. U.S.A., 71, 1695–1700 (1974).
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MINNA, J., COON, H. Human x mouse hybrid cells segregating mouse chromosomes and isozymes. Nature 252, 401–404 (1974). https://doi.org/10.1038/252401a0
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DOI: https://doi.org/10.1038/252401a0
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