Abstract
ORGAN culture of embryonic avian and murine thymus1–5, as well as explants of guinea pig and rabbit thymus6–10, have been observed to produce outgrowths which in some cases were described as predominantly lymphoid in nature, epithelial in other cases. Two publications have described human embryonic thymus in culture, using the plasma clot technique9,10. We have established epithelial outgrowths from explants of human thymic tissue and maintained these cultures for periods up to 3 months.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Auerbach, R., Devl Biol., 3, 366–342 (1961).
Ball, W. D., and Auerbach, R., Expl Cell Res., 20, 245–247 (1960).
Mandel, T., and Russell, P. J., Immunology, 21, 659–674 (1971).
Emmart, E. W., Anat. Rec., 66, No. 1, 59–73 (1936).
Söllström, J. F., and Alm, G. V., Adv. exp. Med. Biol., 29, 35–41 (1973).
Deanesly, R., Q. Jl Microsc. Sci., 72, 247–275 (1929).
Dustin, A. P., and Baillez, G., Bull. Soc. R. Sci. med. nat., Bruxelles (1913).
Murray, R. G., Am. J. Anat., 81, 369–411 (1947).
Van den Tweel, J. C. Thesis, State University of Utrecht, Netherlands (1971).
Törö, I., Röhlrich, P., Olah, I., and Pályi, I., Z. Zellförsch. mikrosk. Anat., 65, 915–929 (1965).
Gelfand, E. W., Baumal, R., Huber, J., Crookston, M., and Shumak, K., New Engl J. Med., 289, 1385–1389 (1973).
Goldstein, G., Nature, 247, 11–14 (1974).
Goldstein, A. L., Guha, A., Zatz, M., Hardy, M., and White, A., Proc. natn. Acad. Sci., U.S.A., 69, 1800–1803 (1972).
Komuro, K., and Boyse, E. A., Lancet, 1, 740–743 (1973).
Komuro, K., and Boyse, E. A., J. exp Med., 138, 479–482 (1973).
Bach, J. F., Papiernik, M., Levasseur, P., Dardenne, M., Barois, A., and LeBrigand, H., Lancet, 2, 1056–1058 (1972).
Bach, J. F., Dardenne, M., Goldstein, A. L., Guha, A., and White, H., Proc. natn Acad. Sci. U.S.A., 68, 2734–2742 (1971).
Mandi, B., and Giant, T., Nature new Biol., 246, 25 (1973).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
PYKE, K., GELFAND, E. Morphological and functional maturation of human thymic epithelium in culture. Nature 251, 421–423 (1974). https://doi.org/10.1038/251421a0
Received:
Issue Date:
DOI: https://doi.org/10.1038/251421a0
This article is cited by
-
Cultivation, characterization and modulation of rat thymic nonlymphoid cells in vitro
Cytotechnology (1991)
-
The growth of nonlymphoid thymic components in vitro: Age-related differences during development
In Vitro Cellular & Developmental Biology (1988)
-
A cloned rat thymic epithelial cell line established from serum-free selective culture
In Vitro Cellular & Developmental Biology (1988)
-
Long-term proliferation of mouse thymic epithelial cells in culture
In Vitro Cellular & Developmental Biology (1986)
-
Cytological characteristics of cultured epithelial cells from thymomas in BUF/Mna rats
Virchows Archiv B Cell Pathology Including Molecular Pathology (1984)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.