Abstract
VIRUS-MEDIATED fusion of somatic cells to produce interspecific cell hybrids has proved to be a most valuable technique for biological research1. Such crosses often undergo an initial fairly random loss of many chromosomes of one parent before karyotypic stability is reached. This property can be used to generate clones of cells each containing a small selection of human chromosomes in a background genotype of some other species. Comparisons of such cell lines provide a form of segregation analysis which can substitute for more traditional genetic methods, particularly in studies on man where classical genetic methodology is difficult to apply.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Harris, H., Cell Fusion (Harvard University Press, Cambridge, 1970).
New Haven Conference: First International Workshop on Human Gene Mapping. Birth Defects: Original Article Series X:3. (The National Foundation, New York, 1974).
Bobrow, M., Madan, K., and Pearson, P. L., Nature new Biol., 238, 122–124 (1972).
Gagne, A., and Laberge, C., Expl Cell Res., 73, 239–242 (1972).
Seabright, M., Lancet, ii, 971–972 (1971).
Sumner, A. T., and Evans, H. L., Expl Cell Res., 81, 223–236 (1973).
Patil, S. R., Merrick, S., and Lubs, H. A., Science, 172, 821–822 (1971).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
BOBROW, M., CROSS, J. Differential staining of human and mouse chromosomes in interspecific cell hybrids. Nature 251, 77–79 (1974). https://doi.org/10.1038/251077a0
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1038/251077a0
This article is cited by
-
Localisation of the human gene encoding the cytoskeletal protein talin to chromosome 9p
Human Genetics (1995)
-
Genetic analysis of transgenome structure and size of chromosome-mediated gene transfer lines
Cell Research (1992)
-
A gene that partially complements xeroderma pigmentosum group A cells maps to human chromosome 8
Somatic Cell and Molecular Genetics (1992)
-
The human cardiac troponin I locus: assignment to chromosome 19p13.2?19q13.2
Human Genetics (1991)
-
Evolutionary conservation of the human homologue of the yeast cell cycle control gene cdc2 and assignment of Cd2 to chromosome 10
Human Genetics (1988)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.