Abstract
WE have recently reported1 the existence of dopamine-sensitive adenyl cyclase (AC) in mouse adrenergic neuroblastoma cells which contain tyrosine hydroxylase (TH) but no choline acetyltransferase (ChA); whereas, nor-adrenaline-sensitive AC at low noradrenaline concentrations becomes apparent in cyclic AMP induced ‘differentiated’ neuroblastoma cells. The presence of acetylcholine receptors in neuroblastoma cells has been demonstrated by Harris and Dennis2. These acetylcholine receptors are blocked by nicotinic and muscarinic inhibitors3. We now report the presence of acetylcholine-sensitive AC in addition to dopamine- and noradrenaline-sensitive AC in inactive neuroblastoma cells which contain neither TH nor ChA, and in cholinergic neuroblastoma cells which contain ChA but no TH. Acetylcholine, dopamine and noradrenaline produced an additive stimulatory effect on AC activity, suggesting that these neurotransmitters act on different receptor sites all of which are linked with AC.
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PRASAD, K., GILMER, K. & SAHU, S. Demonstration of acetylcholine-sensitive adenyl cyclase in malignant neuroblastoma cells in culture. Nature 249, 765–767 (1974). https://doi.org/10.1038/249765a0
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DOI: https://doi.org/10.1038/249765a0
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