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Carbohydrate groups in the major glycoprotein of Rous sarcoma virus

Abstract

GLYCOPROTEINS and other complex carbohydrates on the cell surfaces have been thought to be involved in many biological interactions1. Most enveloped viruses contain at least one glycoprotein component1. In avian sarcoma and leukaemia viruses some of the glycoproteins are located on the surfaces of the particles as projections composed of spikes and knobs2–4. Although their role is not well understood, the glycoproteins are believed to be involved in the determination of host range specificity, induction of neutralising antibody and interference capacity2,5–11. Rous sarcoma virus contains several glycoproteins10–13, and infectious and non-infectious strains of Rous sarcoma virus differ in major glycoprotein components14,15. We report here the results of purification of the major glycoprotein component (g2) of Rous sarcoma virus, its amino acid composition, and the nature of its carbohydrate groups.

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References

  1. Hughes, R. C., Prog. Biophys. molec. Biol., 26, 191–268 (1973).

    Article  Google Scholar 

  2. Bolognesi, D. P., Bauer, H., Gelderblom, H., and Huper, G., Virology, 47, 551–566 (1972).

    Article  CAS  PubMed  Google Scholar 

  3. Bolognesi, D. P., Luftig, R., and Shaper, J. H., Virology, 56, 549–564 (1973).

    Article  CAS  PubMed  Google Scholar 

  4. Rifkin, D. B., and Compans, R. W., Virology, 46, 485–489 (1971).

    Article  CAS  PubMed  Google Scholar 

  5. Vogt, P. K., and Ishizaki, R., Virology, 26, 664–672 (1965).

    Article  CAS  PubMed  Google Scholar 

  6. Vogt, P. K., and Ishizaki, R., Virology, 30, 368–374 (1966).

    Article  CAS  PubMed  Google Scholar 

  7. Ishizaki, R., and Vogt, P. K., Virology, 30, 375–387 (1966).

    Article  CAS  PubMed  Google Scholar 

  8. Bauer, H., and Bolognesi, D. P., Virology, 42, 1113–1126 (1970).

    Article  CAS  PubMed  Google Scholar 

  9. Bauer, H., and Graf, T., Virology, 37, 157–161 (1969).

    Article  CAS  PubMed  Google Scholar 

  10. Duesberg, P. H., Martin, G. S., and Vogt, P. K., Virology, 41, 631–646 (1970).

    Article  CAS  PubMed  Google Scholar 

  11. Tozawa, H., Bauer, H., Graf, T., and Gelderblom, H., Virology, 40, 530–539 (1970).

    Article  CAS  PubMed  Google Scholar 

  12. Fleissner, E., J. Virol., 8, 778–785 (1971).

    CAS  PubMed  PubMed Central  Google Scholar 

  13. Hung, P. P., Robinson, H. L., and Robinson, W. S., Virology, 43, 251–266 (1971).

    Article  CAS  PubMed  Google Scholar 

  14. Scheele, C. M., and Hanafusa, H., Virology, 45, 401–410 (1971).

    Article  CAS  PubMed  Google Scholar 

  15. Robinson, W. S., Hung, P. P., Robinson, H. L., and Ralph, D. D., J. Virol., 6, 695–698 (1970).

    CAS  PubMed  PubMed Central  Google Scholar 

  16. Hobom-Schnegg, B., Robinson, H. L., and Robinson, W. S., J. gen. Virol., 7, 85–93 (1970).

    Article  CAS  PubMed  Google Scholar 

  17. Wang, S., Kothari, R. M., Taylor, M., and Hung, P., Nature new Biol., 242, 133–135 (1973).

    Article  CAS  PubMed  Google Scholar 

  18. Hung, P. P., and Overby, L. R., Biochemistry, 8, 820–828 (1969).

    Article  CAS  PubMed  Google Scholar 

  19. Lee, Y. C., Methods in Enzymology, 28, 63–73 (1972).

    Article  Google Scholar 

  20. Lee, Y. C., and Scocca, J. R., J. biol. Chem., 247, 5753–5759 (1972).

    CAS  PubMed  Google Scholar 

  21. Warren, L., J. biol. Chem., 234, 1971–1975 (1959).

    CAS  PubMed  Google Scholar 

  22. Li, Y. T., and Li, S. C., Methods in Enzymology, 28, 702–713 (1972).

    Article  Google Scholar 

  23. Dorner, F., Scriba, M., and Weil, R., Proc. natn. Acad. Sci. U.S.A., 70, 1981–1985 (1973).

    Article  ADS  CAS  Google Scholar 

  24. Roseman, S., Chem. Phys. Lipids, 5, 270–297 (1971).

    Article  Google Scholar 

  25. Chipowsky, S., Lee, Y. C., and Roseman, S., Proc. natn. Acad. Sci. U.S.A., 70, 2309–2312 (1973).

    Article  ADS  CAS  Google Scholar 

  26. Gottschalk, A., Belyavin, G., and Biddle, F., in Glycoproteins (edit. by Gottschalk, A.), 1082 (Elsevier, Amsterdam, 1972).

    Google Scholar 

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KRANTZ, M., LEE, Y. & HUNG, P. Carbohydrate groups in the major glycoprotein of Rous sarcoma virus. Nature 248, 684–686 (1974). https://doi.org/10.1038/248684a0

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