Abstract
WE have reported that fucosylglycolipid metabolism is markedly altered in cells transformed by oncornavirus1. Transformed cells exhibit a sharp decrease in radioactive fucose incorporation into a chromatographically less mobile fucosylglycolipid, with a corresponding increase in radioactivity in chromatographically more mobile fucosylglycolipids. We describe here further studies of fucosylglycolipid metabolism using normal rat kidney (NRK) cells transformed by a cold-sensitive mutant of murine sarcoma virus (MSV)2. The cell line, designated NRK (MSV-1b), is cold sensitive for maintenance of the transformed phenotype. The cells express the transformed phenotype at the permissive temperature (39° C) but appear phenotypically normal at the non-permissive temperature (33° C). Cells transformed by this mutant are non-producer cells, but contain the MSV genome in a rescuable form. Using these cold-sensitive transformants, we were able to demonstrate, in a temporal fashion, a relationship between altered fucosylglycolipid metabolism and the development of the transformed phenotype.
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References
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STEINER, S., MELNICK, J., KIT, S. et al. Fucosylglycolipids in cells transformed by a temperature-sensitive mutant of murine sarcoma virus. Nature 248, 682–684 (1974). https://doi.org/10.1038/248682a0
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DOI: https://doi.org/10.1038/248682a0
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