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The prevalence of IG translocations and 7q32 deletions in splenic marginal zone lymphoma

Leukemia volume 22, pages 1268–1272 (2008)Cite this article

Splenic marginal zone lymphoma (SMZL) is an uncommon low-grade B-cell lymphoma that has been recognized as a unique clinicopathologic entity in the WHO (World Health Organization) classification.1 Although the clinical, morphological and immunophenotypic features of SMZL are well established, the genetic features of SMZL are not well understood. Characteristic cytogenetic abnormalities that are thought to be primary pathogenetic events have been identified in several mature B-cell neoplasms. Many of these, such as t(11;14)/IGH-CCND1 in mantle cell lymphoma and t(14;18)/IGH-BCL2 in follicular lymphoma, are balanced translocations that juxtapose the immunoglobulin enhancer region with a cellular proto-oncogene, resulting in constitutive activation of the proto-oncogene. However, an analogous cytogenetic abnormality has not been identified in SMZL. On the basis of karyotyping, there have been sporadic reports of balanced translocations in SMZL involving 14q32, 2p11–12 and 22q11, presumably corresponding to IGH, IGK and IGL translocations, respectively. However, the prevalence of these translocations is unknown, and the specific genes involved have rarely been demonstrated. From comparative genomic hybridization, it was found that frequent copy number imbalances in SMZL include gains of the long arm of chromosomes 3 (17–23%) and 12 (14–19%) and loss of the long arm of chromosome 7 (14–21%).2, 3, 4, 5, 6 There has been particular interest in the latter abnormality, and fine mapping has demonstrated that the common deleted region spans 7q31.33–7q33,5 and may even span a narrower region from 7q32.1 to 7q32–3.7 However, its prevalence in SMZL has not been well established.

To determine the prevalence of IG translocations and del(7q) in SMZL, we performed interphase fluorescence in situ hybridization (FISH) on 210 paraffin-embedded SMZL specimens from 210 patients. We also performed karyotypic analysis on 28 cases from this cohort in which fresh or frozen samples were available. All patients had a primary diagnosis of SMZL based on morphology and immunophenotype, using the criteria of the WHO Classification of Haematopoietic and Lymphoid Tissues and consented to research use of their tissue.

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Acknowledgements

This work was supported in part by Grant CA97274 from the National Institutes of Health, Bethesda, MD, USA.

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Authors and Affiliations

  1. Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, USA

    E D Remstein, M Law, P J Kurtin & A Dogan

  2. Department of Genetics and Pathology, Hospital Virgen de la Salud, Toledo, Spain

    M Mollejo

  3. Molecular Pathology Programme, Centro Nacional de Investigaciones Oncológicas (CNIO), Madrid, Spain

    M A Piris

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  1. E D Remstein
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Correspondence to E D Remstein.

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Remstein, E., Law, M., Mollejo, M. et al. The prevalence of IG translocations and 7q32 deletions in splenic marginal zone lymphoma. Leukemia 22, 1268–1272 (2008). https://doi.org/10.1038/sj.leu.2405027

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