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Characterization of three t(3;8)(q27;q24) translocations from diffuse large B-cell lymphomas

Leukemia volume 22, pages 1064–1067 (2008)Cite this article

MYC rearrangements with non-IG partners are observed in about 3% of B-cell non-Hodgkin's lymphomas (NHL). Few studies have been dedicated to this subject until our recent paper1 in which we reported the FISH mapping of 17 MYC-non IG translocations along with the molecular characterization of t(8;9)(q24;p13) translocations identified in two patients. The level of MYC transcript, determined in seven cases, was found to be increased as compared to that of samples without 8q24 rearrangement. Among these 7 cases, two t(3;8)(q27;q24) were detected in patients with germinal center-type diffuse large B-cell lymphoma (GC-DLBCL), UPN602 and UPN1168 (cases no.1 and 2 of this study). FISH mapping of the corresponding breakpoint regions on chromosome 8 showed that they were located 140–370 kb downstream from MYC. On chromosome 3, breakpoints were located in a 65 kb region encompassing BCL6 for case no.1, and 89–200 kb 5′ from BCL6 exon 1 for case no. 2. In the present study, sequencing of the genomic breakpoints was performed by chromosome walking combined with long-distance PCR (Supplementary methods and Supplementary Figure 1).

In case no. 2, chromosome 8 breakpoint was located 369 kb downstream from MYC in a region containing LTR/MaLR-type repetitive sequences (Figure 1b and 2). On chromosome 3, it was located 197 kb 5′ from BCL6, in the vicinity of the BCL6 alternative breakpoint region (ABR).2 Breakpoints could not be defined accurately due to the presence of overlapping nucleotides from chromosomes 3 and 8 sequences. A mutation (A-G transition) was observed at location 189 143 610, together with a possible mutation (G → A transition) at location 189 143 615. In both cases, the breakpoint was within or in close proximity to a RGYW/WRCY motif.

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References

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Acknowledgements

This work was supported by the Ligue Contre le Cancer (Comité de Seine Maritime) and the Fédération des Centres de Lutte contre le Cancer.

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Authors and Affiliations

  1. Groupe d'étude des proliférations lymphoïdes, INSERM U918, IFRMP23, Centre Henri Becquerel, Rouen, France

    P Bertrand, C Maingonnat, J M Picquenot, D Penther, C Maisonneuve, H Tilly & C Bastard

  2. INSERM U563, Centre de Physiopathologie, Toulouse, France

    N Dastugue & L Ysebaert

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  1. P Bertrand
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  2. C Maingonnat
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Correspondence to P Bertrand.

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Supplementary Information accompanies the paper on the Leukemia website (http://www.nature.com/leu)

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Bertrand, P., Maingonnat, C., Picquenot, J. et al. Characterization of three t(3;8)(q27;q24) translocations from diffuse large B-cell lymphomas. Leukemia 22, 1064–1067 (2008). https://doi.org/10.1038/sj.leu.2404999

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