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Stem Cells

Tissue repair using allogeneic mesenchymal stem cells for hemorrhagic cystitis, pneumomediastinum and perforated colon

Abstract

Mesenchymal stem cells (MSC) possess anti-inflammatory properties and participate in tissue repair. We used MSC to heal therapy-induced tissue toxicity. Ten consecutive patients, treated with MSC due to tissue toxicity following allogeneic hematopoietic stem cell transplantation, (ASCT) were included. Their median age was 48 (13–64) years. Seven had hemorrhagic cystitis grades 2–5, two had pneumomediastinum and one had perforated colon and peritonitis. MSC donors were mainly third-party, HLA-mismatched (n=11), HLA-haploidentical (n=3) and, in two cases, the HLA-identical ASCT sibling donors. MSC were given intravenously, the median cell dose was 1.0 (range 0.7–2) × 106/kg. In five patients, the severe hemorrhagic cystitis cleared after MSC infusion. Gross hematuria disappeared after median 3 (1–14) days. Two patients had reduced transfusion requirements after MSC infusion, but died of multiorgan failure. In one of them, MSC donor DNA was demonstrated in the urinary bladder. In two patients, pneumomediastinum disappeared after MSC infusions. A patient with steroid-resistant graft-versus-host disease of the gut experienced perforated diverticulitis and peritonitis that was reversed twice by MSC. MSC is a novel treatment for therapy-induced tissue toxicity.

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Acknowledgements

We thank Maivor Hoglund-Lindecrantz for data collection and Inger Hammarberg for secretarial assistance. This study was supported by grants from the Swedish Cancer Society (0070-B02–16XAC, 4562-B02–02XBB), the Children's Cancer Foundation (2000/067, 01/039), the Swedish Research Council (K2003–32X-05971–23A, K2003–32XD-14716–01A), the Cancer Society in Stockholm, the Cancer- and Allergy Foundation, and Karolinska Institutet.

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Correspondence to O Ringdén.

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Ringdén, O., Uzunel, M., Sundberg, B. et al. Tissue repair using allogeneic mesenchymal stem cells for hemorrhagic cystitis, pneumomediastinum and perforated colon. Leukemia 21, 2271–2276 (2007). https://doi.org/10.1038/sj.leu.2404833

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