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  • Letter to the Editor
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Reply to the c-kit(CD117) sequence variation M541L, but not N564K, is frequent in the general population, and is not associated with CML in Caucasuans by Stefan Kruger et al.

Leukemia volume 20, page 356 (2006)Cite this article

We welcome the comments, and appreciate the interest in our work by Kruger et al.,1 and we thank the Editor for the opportunity to respond to their observations. Their data and comments contain some interesting data but also some generally misleading viewpoints. In our report, we specifically highlighted the critical impact that polymorphism in the c-kit gene can exert on the clinical phenotype. The single nucleotide change (ATG → CTG) in codon 541 is an example of a clearly single nucleotide polymorphism (SNP) of M541L, as described in our earlier report.2

SNPs were detected in five of the six chronic myelogenous leukemia (CML) patients as shown in Table 2 in that earlier report.2 This was because the DNA of the skin and hair root had the same variation. The same variation was found only in bone marrow cells of blastic crisis (BC) in the case of the remaining one patient (Case No. 2). This means that M541L could not be the cause of chronic-phase CML. N564K was also found only in the bone marrow cells of BC of that patient (Case No. 1). This means that N564K also could not be the cause of the chronic phase of CML.

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References

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Authors and Affiliations

  1. Division of Hematology, Department of Internal Medicine, Nippon Medical School, Tokyo, Japan

    K Inokuchi, H Yamaguchi, M Tarusawa, M Futaki, S Tanosaki & K Dan

  2. Department of Molecular Biology, Nippon Medical School, Tokyo, Japan

    M Tarusawa & H Hanawa

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  1. K Inokuchi
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  2. H Yamaguchi
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  3. M Tarusawa
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  4. M Futaki
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  5. H Hanawa
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  6. S Tanosaki
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  7. K Dan
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Inokuchi, K., Yamaguchi, H., Tarusawa, M. et al. Reply to the c-kit(CD117) sequence variation M541L, but not N564K, is frequent in the general population, and is not associated with CML in Caucasuans by Stefan Kruger et al.. Leukemia 20, 356 (2006). https://doi.org/10.1038/sj.leu.2404039

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