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Mobilisation with G-CSF in healthy donors promotes a high but temporal deregulation of genes

Leukemia volume 19, pages 1088–1091 (2005)Cite this article

TO THE EDITOR

Over recent years, the use of peripheral blood (PB) instead of bone marrow (BM) as a source for stem cells has been increasingly employed both in the autologous and in the allogeneic transplant setting. The advantages of peripheral blood stem cells (PBSC) include easier harvesting of stem cells, avoidance of anaesthesia and hospitalisation as well as improved yield of progenitor cells, and a more rapid and robust engraftment. However, it should also be taken into account that PBSC mobilisation requires the administration of haematopoietic cytokines (ie granulocyte colony-stimulating factor (G-CSF)) over several days, which stimulate proliferation and differentiation of myeloid cell lineage.1 Extensive experience so far accumulated with G-CSF in patients being treated for neutropenia as well as in patients undergoing autologous stem cell mobilisation indicates that it is a safe procedure. Nevertheless, the parameters used for the safety evaluation have been rather crude and mainly focus on morphological and cytogenetic changes in haemopoietic cells.2 Therefore, variations in other cell systems may have gone undetected. This is particularly relevant when considering the use of G-CSF in healthy volunteer individuals.

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References

  1. Rowley SD, Donaldson G, Lilleby K, Bensinger WI, Appelbaum FR . Experiences of donors enrolled in a randomized study of allogeneic bone marrow or peripheral blood stem cell transplantation. Blood 2001; 97: 2541–3051.

    Article  CAS  Google Scholar 

  2. Steidl U, Kronenwett R, Rohr UP, Fenk R, Kliszewski S, Maercker C et al. Gene expression profiling identifies significant differences between the molecular phenotypes of bone marrow-derived and circulating human CD34+ hematopoietic stem cells. Blood 2002; 99: 2037–2044.

    Article  CAS  Google Scholar 

  3. Valk PJ, Verhaak RG, Beijen MA, Erpelinck CA, Barjesteh van Waalwijk van Doorn-Khosrovani S, Boer JM et al. Prognostically useful gene-expression profiles in acute myeloid leukemia. N Engl J Med 2004; 350: 1617–1628.

    Article  CAS  Google Scholar 

  4. Papayannopoulou T . Current mechanistic scenarios in hematopoietic stem/progenitor cell mobilisation. Blood 2004; 103: 1580–1585.

    Article  CAS  Google Scholar 

  5. Yang FC, Atkinson SJ, Gu Y, Borneo JB, Roberts AW, Zheng Y et al. Rac and Cdc42 GTPases control hematopoietic stem cell shape, adhesion, migration, and mobilisation. Proc Natl Acad Sci USA 2001; 98: 5614–5618.

    Article  CAS  Google Scholar 

  6. Mossessova E, Bickford LC, Goldberg J . SNARE selectivity of the COPII coat. Cell 2003; 114: 483–495.

    Article  CAS  Google Scholar 

  7. Clemens MJ . Targets and mechanisms for the regulation of translation in malignant transformation. Oncogene 2004; 23: 3180–3188.

    Article  CAS  Google Scholar 

  8. Franzke A, Piao W, Lauber J, Gatzlaff P, Konecke C, Hansen W et al. G-CSF as immune regulator in T cells expressing the G-CSF receptor: implications for transplantation and autoimmune disease. Blood 2003; 15: 734–739.

    Article  Google Scholar 

Download references

Acknowledgements

This study was partially supported by ‘Ministerio de Ciencia y Tecnología’ grant (SAF2001-1687) and Spanish FIS grants (02/1358 and 03/0920), partially financed by ‘Fondos Estructurales FEDER’ from the European Union. NCG was partially supported by a grant from ‘Fundación Española de Hematología y Hemoterapia’ and JLG by a grant from Spanish FIS (01/3153).

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Authors and Affiliations

  1. Servicio de Hematología, Hospital Universitario de Salamanca & Centro de Investigación del Cáncer (CIC), Salamanca, Spain

    J M Hernández, C Castilla, N C Gutiérrez, M Delgado, J L García, E M Ocio, M C del Cañizo & J F San Miguel

  2. Unidad de Bioinformática, Centro de Investigación del Cáncer (CIC) Universidad de Salamanca-CSIC, Salamanca, Spain

    J de las Rivas

  3. Unidad de Genómica y Proteómica, Centro de Investigación del Cáncer (CIC) Universidad de Salamanca-CSIC, Salamanca, Spain

    J M Hernández, N C Gutiérrez, I M Isidro, E Fermiñán, J L García & J F San Miguel

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  1. J M Hernández
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Correspondence to J M Hernández.

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Hernández, J., Castilla, C., Gutiérrez, N. et al. Mobilisation with G-CSF in healthy donors promotes a high but temporal deregulation of genes. Leukemia 19, 1088–1091 (2005). https://doi.org/10.1038/sj.leu.2403753

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