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Rapid reconstitution of dendritic cells after allogeneic transplantation of CD133+ selected hematopoietic stem cells

Leukemia volume 19, pages 161–165 (2005)Cite this article

TO THE EDITOR

Throughout the last decade, monoclonal antibodies against the CD34 antigen have been used to mark and select hematopoietic stem cells. In the meantime, a new antigen initially called AC-133 and now referred to as prominin-1 or CD133,1 a member of the family of pentaspan transmembrane glycoproteins, has been shown to be expressed on a subpopulation of CD34+ hematopoietic stem cells. CD133+ cells in the peripheral blood have been shown to comprise early stem cells with substantial NOD/SCID mouse repopulating capacity and potential plasticity.2 Large-scale immunomagnetic selection using a monoclonal anti-CD133 antibody allows to enrich sufficient numbers of hematopoietic progenitor cells to perform hematopoietic stem cell transplantation (HSCT). We performed a feasibility trial in 10 adult patients with leukemia to test whether sustained engraftment can be achieved with allogeneic CD133+ purified stem cells. Secondary end point was the kinetics of donor chimerism in myeloid and lymphoid cells as well as in blood dendritic cells (DCs).

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Acknowledgements

We thank Kristina Hölig, Verona Schwarze, Anja Maiwald, Anja Weisske and Diana Döhler for PBSC preparation and CD133+ immunomagnetic selection. We also thank Manuela Neumann and Cornelia Grosse for cell sorting and chimerism analysis. This work was supported in part by the Deutsche Krebshilfe Grant 70-3197 (MB and CT) and by Miltenyi Biotec, Bergisch Gladbach, Germany.

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  1. Medizinische Klinik und Poliklinik I, University Hospital, Dresden, Germany

    M Bornhäuser, L Eger, U Oelschlaegel, S Auffermann-Gretzinger, A Kiani, J Schetelig, T Illmer, M Schaich, D Corbeil, C Thiede & G Ehninger

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  1. M Bornhäuser
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  2. L Eger
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Correspondence to M Bornhäuser.

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Bornhäuser, M., Eger, L., Oelschlaegel, U. et al. Rapid reconstitution of dendritic cells after allogeneic transplantation of CD133+ selected hematopoietic stem cells. Leukemia 19, 161–165 (2005). https://doi.org/10.1038/sj.leu.2403563

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