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Quantitative analysis of chimerism after allogeneic bone marrow transplantation using immunomagnetic selection and fluorescent microsatellite PCR

Leukemia volume 17, pages 247–251 (2003)Cite this article

A series of recent publications has highlighted the importance of changing patterns of donor/recipient chimerism kinetics in the evolution of both relapse and rejection.1,2,3,4,5,6,7 Yet it remains difficult to define consistent patterns between these various reports. In part this may result from the use of differing methods of analysis (and inherent sensitivity) between centres and inconsistency between the times after transplant at which analyses are performed. Further diversity is added by the range of conditions transplanted, and conditioning protocols and regimens of GVHD prophylaxis employed.

The editors of Leukemia have therefore sought to standardise the technological approaches used and to provide a framework for the comparison of diagnostic results through their Round-Table Debate on chimerism, which began in 1999.8 As part of this continuing debate,9,10 we present technical details of the assay developed in our laboratory for quantitative analysis of chimerism.

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Authors and Affiliations

  1. Department of Pathology and Microbiology, University of Bristol, Bristol, UK

    J P Hancock, N J Goulden, A Oakhill & C G Steward

  2. Bristol Royal Hospital for Children, Bristol, UK

    N J Goulden, A Oakhill & C G Steward

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  1. J P Hancock
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  2. N J Goulden
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  3. A Oakhill
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  4. C G Steward
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Hancock, J., Goulden, N., Oakhill, A. et al. Quantitative analysis of chimerism after allogeneic bone marrow transplantation using immunomagnetic selection and fluorescent microsatellite PCR. Leukemia 17, 247–251 (2003). https://doi.org/10.1038/sj.leu.2402759

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