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Summary: Reports on quantitative analysis of chimerism after allogeneic stem cell transplantation by PCR amplification of microsatellite markers and capillary electrophoresis with fluorescence detection

  • T Lion1
  • on behalf of the participants of the current round of the Debate

Leukemia volume 17, pages 252–254 (2003)Cite this article

The reports from a number of European diagnostic centers presented in this round of the Debate on Chimerism Testing reveal several technical aspects of great relevance to the clinical monitoring of post-transplant chimerism. The main advantages of automated fluorescence-based detection of microsatellite markers over the use of conventional polyacrylamide gel electrophoresis (PAGE) include greater precision and easier performance of quantitative analysis, reduced manual handling of PCR products, and higher sensitivity. Important features and potential problems of the technique discussed in the contributions to the debate are summarized below.

Only a limited number of highly polymorphic microsatellite loci needs to be tested to provide an informative marker in virtually all donor/recipient constellations. In matched sibling and haploidentical transplants more markers are generally required in order to differentiate between donor and recipient cells as compared to the unrelated setting. The screening panels used at the above centers include 6–15 microsatellite markers. In most instances, genotyping with 3–5 markers is sufficient to reveal allelic differences between recipient- and donor-derived cells suitable for the analysis of chimerism.

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References

  1. Thiede, C, Bornhauser, M, Oelschlager, U, Brendel, C, Leo, R, Daxberger, H, Mohr, B, Florek, M, Kroschinsky, F, Geissler, G, Naumann, R, Ritter, M, Prange-Krex, G, Lion, T, Neubauer, A & Ehninger, G Sequential monitoring of chimerism and detection of minimal residual disease after allogeneic blood stem cell transplantation (BSCT) using multiplex PCR amplification of short tandem repeat-markers. Leukemia, (2001). 15, 293–302.

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  1. Children's Cancer Research Institute, Vienna, Austria

    T Lion

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on behalf of the participants of the current round of the Debate

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Lion, T., on behalf of the participants of the current round of the Debate. Summary: Reports on quantitative analysis of chimerism after allogeneic stem cell transplantation by PCR amplification of microsatellite markers and capillary electrophoresis with fluorescence detection. Leukemia 17, 252–254 (2003). https://doi.org/10.1038/sj.leu.2402753

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