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Differential bone marrow aspirate DNA yields from commercial extraction kits

Leukemia volume 16, pages 1865–1866 (2002)Cite this article

Extraction of DNA from bone marrow slides for molecular biology studies is a standard technique for which multiple commercial DNA extraction kits are available. Slides of bone marrow and other tissues may also be used as a DNA source for molecular epidemiological studies.1 Adequate amounts of DNA may be obtained from both stained and unstained slides,2 DNA may be obtained from specimens archived for decades,3 and different phenol–chloroform extraction procedures give varied DNA yields.4 Since phenol–chloroform reactions are time-consuming and expose laboratory personnel to toxic reagents, research laboratories most often use commercial kits for DNA extraction. Verhagen et al5 have shown that DNA yields adequate for Southern blotting may be obtained from bone marrow specimens with the Qiagen column, a common commercial DNA extraction kit.

Commercial DNA extraction kits involve various techniques for DNA purification. All kits employ cell lysis and protein denaturations steps to obtain DNA for purification. Some kits use a silica-gel based column to bind DNA, followed by serial washes to remove non-DNA cell contents and DNA elution in a low salt buffer (Qiagen). Other kits use a ‘salting out’ procedure with alcohol extraction (Puregene) to isolate and purify DNA.

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Authors and Affiliations

  1. The Center for Clinical Epidemiology and Biostatistics, University of Pennsylvania, Philadelphia, PA, USA

    R Aplenc, J Swoyer, B Manke & T Rebbeck

  2. The Children's Hospital of Philadelphia, Philadelphia, PA, USA

    R Aplenc & E Orudjev

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  1. R Aplenc
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  2. E Orudjev
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  3. J Swoyer
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  4. B Manke
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  5. T Rebbeck
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Aplenc, R., Orudjev, E., Swoyer, J. et al. Differential bone marrow aspirate DNA yields from commercial extraction kits. Leukemia 16, 1865–1866 (2002). https://doi.org/10.1038/sj.leu.2402681

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  • DOI: https://doi.org/10.1038/sj.leu.2402681

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