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A new recurrent and specific cryptic translocation, t(5;14)(q35;q32), is associated with expression of the Hox11L2 gene in T acute lymphoblastic leukemia

Leukemia volume 15, pages 1495–1504 (2001)Cite this article

Abstract

FISH identified a cryptic t(5;14)(q35;q32) in T acute lymphoblastic leukemia (ALL), whereas it was not observed in B ALL samples. This translocation is present in five out of 23 (22%) children and adolescents with T ALL tested. RanBP17, a gene coding for a member of the importin β protein family, and Hox11Like2, an orphan homeobox gene were mapped close to the chromosome 5 breakpoints and CTIP2, which is highly expressed during normal T cell differentiation, was localized in the vicinity of the chromosome 14 breakpoints. The Hox11L2 gene was found to be transcriptionally activated as a result of the translocation, probably under the influence of CTIP2 transcriptional regulation elements. These data establish the t(5;14)(q35;q32) as a major abnormality, and Hox11 family member activation as an important pathway in T ALL leukemogenesis.

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Acknowledgements

This work was supported by INSERM and the Ligue Nationale Contre le Cancer. Thomas Mercher and Richard Monni are recipients of fellowships from the Ministère de l'Education Nationale. Florence Nguyen Khac is supported by the Académie Nationale de Médecine. We thank W Vainschenker for providing us with the M70E cell line.

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Authors and Affiliations

  1. U434 INSERM-CEPH and SD401 No. 434 CNRS, Paris, France

    OA Bernard, M Busson-LeConiat, M Mauchauffé, V Della Valle, R Monni, F Nguyen Khac, T Mercher, V Penard-Lacronique & R Berger

  2. Service d'Hématologie Biologique, Hôpital Trousseau, Paris, France

    P Ballerini

  3. CEPH, Paris, France

    P Pasturaud & L Gressin

  4. Génoscope, Evry, France

    R Heilig

  5. Laboratoire Central d'Hématologie, Hôpital Saint-Louis, Paris, France

    M-T Daniel

  6. Laboratoire d'Hématologie, Hôpitaux Universitaires de Strasbourg, France

    M Lessard

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Bernard, O., Busson-LeConiat, M., Ballerini, P. et al. A new recurrent and specific cryptic translocation, t(5;14)(q35;q32), is associated with expression of the Hox11L2 gene in T acute lymphoblastic leukemia. Leukemia 15, 1495–1504 (2001). https://doi.org/10.1038/sj.leu.2402249

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