Abstract
Integration of morphological and immunophenotypic data is critical in achieving diagnosis accuracy and minimising interobserver interpretative discrepancies. The aim of this work was to compare the immunophenotype and the morphology of chronic lymphocytic leukaemia and mantle cell lymphoma, to help in the differential diagnosis of CD5 positive monoclonal B cells. Frozen/thawed samples from 91 patients were analysed retrospectively. Fresh samples from 17 mixed/atypical CLL and 13 MCL were tested to corroborate the results. Markers were analysed as percentage (%) of positive B lymphocyte subpopulation, and in terms of median fluorescence intensity (MFI). Matutes's CLL score clearly allowed distinguishing between classical CLL on the one hand, and atypical CLL and MCL on the other hand. The percentage of CD54-positive cells and the median fluorescence intensity of CD20 and CD54 were the only parameters which were significantly higher in MCL than in atypical CLL (P < 0.05), allowing an immunological distinction between these two entities. Nevertheless, due to a quenching problem when using CD20 and CD54 together, and because CD18 showed a statistically different expression between classical and atypical CLL, the combination of CD18/CD54 has been preferred and showed a different pattern in the three entities. Immunophenotyping could be helpful in the differential diagnosis of CD5-positive B cell chronic lymphoproliferative disorders with atypical features that do not fit exactly into any of the morphologic proposed groups.
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References
Bennett JM, Catovsky D, Daniel MT, Flandrin G, Galton DAG, Gralnick HR, Sultan C . The French–American–British (FAB) cooperative study group: proposals for the classification of chronic (mature) B and T lymphoid leukemias J Clin Pathol 1989 42: 567–584
Matutes E, Owusuankomah K, Morilla R, Marco JG, Houlihan A, Que TH, Catovsky D . The immunological profile of B-cell disorders and proposal of a scoring system for the diagnosis of CLL Leukemia 1994 8: 1640–1645
Working Formulation Classification . Non-Hodgkin's lymphoma pathologic classification project. National Cancer Institute sponsored study of classifications of non-Hodgkin's lymphomas: summary and description of a Working Formulation for clinical usage Cancer 1982 49: 2112–2135
Valet GK, Hökkes HG . Automated classification of patients with chronic lymphocytic leukemia and immunocytoma from flow cytometric three color immunophenotypes Cytometry 1997 30: 275–288
Bray RA . Flow cytometry crossmatching for solid organ transplantation Meth Cell Biol 1994 41: 103–119
Keeney M, Gratama JW, Chin-Yee IH, Sutherland DR . Isotype controls in the analysis of lymphocytes and CD34+ stem and progenitor cells by flow cytometry – time to let go Cytometry 1998 34: 280–283
Shapiro HM . Practical Flow Cytometry, 3rd edn Wiley-Liss: New-York 1995
Deneys V, Thiry V, Hougardy N, Mazzon AM, Leveugle P, De Bruyère M . Impact of cryopreservation on B cell chronic lymphocytic leukaemia phenotype J Immunol Meth 1999 228: 13–21
Eisterer W, Hilbe W, Stauder R, Bechter O, Fend F, Thaler J . An aggressive subtype of B-CLL is characterized by strong CD44 expression and lack of CD11c Br J Haematol 1996 93: 661–669
Domingo A, Gonzalez Barca E, Castellsague X, Fernandez Sevilla A, Granena A, Crespo N, Ferran C . Expression of adhesion molecules in 113 patients with B-cell chronic lymphocytic leukemia: relationship with clinico-prognostic features Leuk Res 1997 21: 67–73
Csanaky G, Matutes E, Vass JA, Morilla R, Catovsky D . Adhesion receptors on peripheral blood leukemic B cells. A comparative study on B cell chronic lymphocytic leukemia and related lymphoma/leukemias Leukemia 1997 11: 408–415
Sembries S, Pajl H, Stilgenbauer S, Dohner H, Schriever F . Reduced expression of adhesion molecules and cell signaling receptors by chronic lymphocytic leukemia cells with 11q deletion Blood 1999 93: 624–631
Criel A, Michaux L, de Wolf-Peeters C . The concept of typical and aytpical chronic lymphocytic leukemia Leuk Lymphoma 1999 33: 33–45
Moreau E, Matutes E, a'Hern RP, Morilla AM, Morilla RM, Owusu-Ankomah KA, Seon BK, Catovsky D . Improvement of the chronic lymphocytic leukemia scoring system with the monoclonal antibody SN8 (CD79b) Am J Clin Pathol 1997 108: 378–382
Mufti GJ, Flandrin G, Schaefer HE, Sandberg AA, Kanfer EJ . An Atlas of Malignant Haematology, Cytology, Histology and Cytogenetics Martin Dunitz Ltd: London 1997
Jacob MC, Agrawal S, Chaperot L, Giroux C, Gressin R, Le Marc'Hadour F, Favre M, Sotto JJ, Bensa JC, Plumas J . Quantification of cellular adhesion molecules on malignant B cells from non-Hodgkin's lymphoma Leukemia 1999 13: 1428–1433
Barclay AN, Brown MH, Law SKA, McKnight AJ, Tomlinson MG, van der Merwe PA . The Leucocyte Antigen Facts Book Academic Press: San Diego 1997
Roos E . Adhesion molecules in lymphoma metastasis Cancer Metast Rev 1991 10: 33–48
Koopman G, Parmentier HK, Schuurman HJ, Newman W, Meijer CJLM, Pals ST . Adhesion of human B cells to follicular dendritic cells involves both the lymphocyte function-associated antigen 1/intercellular adhesion molecule 1 and very late antigen 4/vascular cell adhesion molecule 1 pathways J Exp Med 1991 173: 1297–1304
Petrash S, Kosco M, Schmitz, J, Wacker HH, Brittinger G . Follicular dendritic cells in non-Hodgkin's lymphoma express adhesion molecules complementary to ligands on neoplastic B cells Br J Haematol 1992 82: 695–700
Burton GF, Conrad DH, Skazal AK, Tew JG . Follicular dendritic cells and B cell costimulation J Immunol 1993 150: 31–38
Lucio PJMDS, Faria MTSD, Pinto AMC, da Silva MRLG, Correia MEMRR, da Costa RJSM, Parreira ABDS . Expression of adhesion molecules in chronic B-cell lymphoproliferative disorders Haematologica 1998 83: 104–111
Woesner S, Asensio A, Florensa L, Pedro C, Besses C, Sanssabrafen J . Expression of lymphocyte function-associated antigen (LFA)-1 in B-cell chronic lymphocytic leukemia Leuk Lymphoma 1994 13: 457–461
Bubien JK, Zhou LJ, Bell PD, Frizzell RA, Tedder TF . Transfection of the CD20 cell surface molecule into ectopic cell types generates a Ca2+ conductance found constitutively in B lymphocytes J Cell Biol 1993 121: 1121–1132
Kanzaki H, Shibata H, Mogami H, Kojima I . Expression of calcium-permeable cation channel CD20 accelerates progression through the G1 phase in Balb/c 3T3 cells J Biol Chem 1995 270: 13099–13104
Ginaldi L, De Martinis M, Matutes, E, Farahat N, Morilla R, Catovsky D . Levels of expression of CD19 and CD20 in chronic B cell leukaemias J Clin Pathol 1998 51: 364–369
McCarron KF, Hammel JP, Hsi ED . Usefulness of CD79b expression in the diagnosis of B-cell chronic lymphoproliferative disorders Am J Clin Pathol 2000 113: 805–813
D'Arena G, Musto P, Cascavilla N, Dell'Olio M, Di Renzo N, Carotenuto R . Quantitative flow cytometry for the differential diagnosis of leukemic B-cell chronic lymphoproliferative disorders Am J Hematol 2000 64: 275–281
Bell PB, Rooney N, Bosanquet AG . CD79a detected by ZL7.4 separates chronic lymphocytic leukemia from mantle cell lymphoma in the leukemic phase Cytometry 1999 38: 102–105
Levy V, Ugo V, Delmer A, Tang R, Ramond S, Perrot JY, Vrhovac R, Marie JP, Zittoun R, Ajchenbaum-Cymbalista F . Cyclin D1 overexpression allows identification of an agressive subset of leukemic lymphoproliferative disorder Leukemia 1999 13: 1343–1351
Rosenwald A, Ott G, Kruldiek AK, Dreyling MH, Katzenberger T, Kalla J, Roth S, Ott MM, Muller-Hermelinck HR . A biological role for deletions in chromosomal band 13q14 in mantle cell and peripheral T-cell lymphomas Gene Chromosome Cancer 1999 26: 210–214
Schaffner C, Stilgenbauer S, Rappold GA, Dohner H, Lichter P . Somatic ATM mutations indicate a pathogenic role of ATM in B-cell chronic lymphocytic leukemia Blood 1999 94: 748–753
Monni O, Zhu Y, Franssila K, Oinonen R, Hoglund P, Elonen E, Joensuu H, Knuutila S . Molecular characterization of deletion at 11q22.1–23.3 in mantle cell lymphoma Br J Haematol 1999 104: 665–671
Stilgenbauer S, Winkler D, Ott G, Schaffner C, Leupolt E, Bentz M, Moller P, Muller-Hermelinck HK, James MR, Lichter P, Dohner H . Molecular characterization of 11q deletions points to a pathogenic role of the ATM gene in mantle cell lymphoma Blood 1999 94: 3262–3264
Schaffner C, Idler I, Stilgenbauer S, Dohner H, Lichter P . Mantle cell lymphoma is characterized by inactivation of the ATM gene Proc Natl Acad Sci USA 2000 97: 2773–2778
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The authors wish to thank the Salus Sanguinis Foundation for financial supaport, and Mrs Valérie Etienne for technical assistance.
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Deneys, V., Michaux, L., Leveugle, P. et al. Atypical lymphocytic leukemia and mantle cell lymphoma immunologically very close: flow cytometric distinction by the use of CD20 and CD54 expression. Leukemia 15, 1458–1465 (2001). https://doi.org/10.1038/sj.leu.2402200
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DOI: https://doi.org/10.1038/sj.leu.2402200