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Lymphoma

Establishment of a follicular lymphoma cell line (FLK-1) dependent on follicular dendritic cell-like cell line HK

Leukemia volume 15, pages 148–156 (2001)Cite this article

Abstract

A novel cell line, FLK-1, was established from bone marrow cells of a patient with follicular lymphoma by means of co-culture with follicular dendritic cell (FDC)-like cell line HK. Immunophenotypic analysis showed that FLK-1 expressed CD10, CD19, CD20, CD38, IgG and HLA-DR, which is a typical feature of germinal center B cells. Cytogenetic analysis of FLK-1 demonstrated t(14;18)(q32;q21) translocation involving BCL2 and immunoglobulin heavy chain genes. Especially noteworthy is that the growth of FLK-1 was found to be dependent on a FDC line, HK. When HK cells were removed from the culture, FLK-1 cells stopped growing and eventually died. An apoptotic mechanism appeared to be involved as indicated by the presence of chromosome condensation and DNA ladder formation. The culture experiment using micropore membranes showed that soluble factor(s) of HK cells supported the growth, while direct cell-to-cell contact appeared to be necessary for long-term cell proliferation. These findings suggest the importance of the micro-environment for follicular lymphoma cells to grow. The FLK-1 cell line may thus prove to be useful for studying the growth mechanism of follicular lymphoma and provide new insights into the pathogenesis of follicular lymphoma.

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Acknowledgements

The authors thank M Ando, T Kobayashi and H Ishida for their technical assistance. This research was supported in part by a Grant-in-aid from the Ministry of Health and Welfare and a Grant-in-aid from the Ministry of Education, Science and Culture of Japan.

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Authors and Affiliations

  1. Department of Hematology and Chemotherapy, Aichi Cancer Center Hospital, Nagoya, Japan

    Y Kagami & Y Morishima

  2. Laboratory of Cellular Immunology, Alton Ochsner Medical Foundation, New Orleans, LA, USA

    J Jung & YS Choi

  3. Otsuka Assay Laboratory, Tokushima, Japan

    K Osumi

  4. Department of Pathology, Aichi Cancer Center Hospital, Nagoya, Japan

    S Nakamura

  5. Department of Molecular Medicine, Aichi Cancer Center Research Institute, Nagoya, Japan

    M Seto

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Kagami, Y., Jung, J., Choi, Y. et al. Establishment of a follicular lymphoma cell line (FLK-1) dependent on follicular dendritic cell-like cell line HK. Leukemia 15, 148–156 (2001). https://doi.org/10.1038/sj.leu.2402002

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