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Minimal Residual Disease

Immunoglobulin κ gene rearrangements between the κ deleting element and Jκ recombination signal sequences in acute lymphoblastic leukemia and normal hematopoiesis

Leukemia volume 14, pages 671–674 (2000)Cite this article

Abstract

The κ deleting element (κDE) located 24 kb downstream of the Cκ gene segment mediates the deletion of Cκ and the Jκ-Cκ Intron enhancer, which results in allelic exclusion of the immunoglobulin κ light chain locus. We here report that the κDE can recombine to each recombination signal sequence (RSS) flanking Jκ1 to Jκ5 in normal hematopoiesis. Moreover, usage of the JκRSS-κDE junctional sequence allows the detection of minimal residual disease in acute lymphoblastic leukemia.

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Acknowledgements

This work was supported by grants from the Deutsche Krebshilfe and the Deutsche Forschungsgemeinschaft to CRB. The authors gratefully acknowledge Prof W-D Ludwig for immunophenotype analysis, Dr S Yokota and Dr JWG Janssen for helpful discussions, D Erz for technical assistance, A Jordan for secretarial help, and the participants of the German ALL-BFM Study Group for their continuous cooperation.

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Authors and Affiliations

  1. Institute of Human Genetics, University of Heidelberg, Heidelberg, Germany

    T Seriu, TE Hansen-Hagge, Y Stark & CR Bartram

  2. Department of Internal Medicine III, Kyoto Prefectural University of Medicine, Kyoto, Japan

    T Seriu

  3. Department of Dermatology, Faculty of Medicine-Charité, Humboldt University, Berlin, Germany

    TE Hansen-Hagge

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  1. T Seriu
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Seriu, T., Hansen-Hagge, T., Stark, Y. et al. Immunoglobulin κ gene rearrangements between the κ deleting element and Jκ recombination signal sequences in acute lymphoblastic leukemia and normal hematopoiesis. Leukemia 14, 671–674 (2000). https://doi.org/10.1038/sj.leu.2401745

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