Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Original Manuscript
  • Published:

Normal Hemopoiesis and Stemcellology

CD34high+CD38low/− cells generated in a xenogenic coculture system are capable of both long-term hematopoiesis and multiple differentiation

Abstract

CD34+ cells isolated from human umbilical cord blood (HUCB) are thought to have potential in clinical applications such as transplantation and gene therapy. Recently, we developed a xenogenic coculture system involving HUCB-CD34+ cells and murine bone marrow stromal cells, HESS-5 cells, in combination with human interleukin-3 and stem cell factor. Under these xenogenic coculture conditions, the numbers of CD34high+ cells and primitive progenitor cells, such as CD34high+CD38low/− cells and high proliferative potential colony-forming cells (HPP-CFCs), increased dramatically by a factor of 102.1, 66.5 and 104.9, respectively. In the present study, we used a secondary culture of B progenitor cells and long-term culture (LTC)-initiating cells to characterize and compare the progenitor capability of re-isolated CD34high+CD38low/− cells, which have been identified as one of the most primitive progenitor cells, with that of freshly isolated CD34high+CD38low/− cells. Compared with freshly isolated CD34high+CD38low/− cells, the re-isolated CD34high+CD38low/− cells were equally as capable of proliferating and differentiating into myeloid and B progenitor cells. No significant differences were observed in the frequency of LTC-initiating cells in the re-isolated CD34high+CD38low/− cells compared with that in freshly isolated CD34high+CD38low/− cells. Furthermore, the re-isolated CD34high+CD38low/− cells were capable of long-term reconstitution and multiple differentiation in non-obese diabetic mice with severe combined immunodeficiency disease (NOD/SCID mice). The results demonstrate that this xenogenic coculture system can be used for successful in vitro expansion of HUCB-progenitor cells that possess the capability for both long-term hematopoiesis as well as multipotent differentiation into myeloid and lymphoid cells both in vivo and in vitro.

This is a preview of subscription content, access via your institution

Access options

Rent or buy this article

Prices vary by article type

from$1.95

to$39.95

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tsuji, T., Itoh, K., Nishimura-Morita, Y. et al. CD34high+CD38low/− cells generated in a xenogenic coculture system are capable of both long-term hematopoiesis and multiple differentiation. Leukemia 13, 1409–1419 (1999). https://doi.org/10.1038/sj.leu.2401507

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/sj.leu.2401507

Keywords

This article is cited by

Search

Quick links