Abstract
A rapid and simple multiplex polymerase chain reaction (PCR) is described that is capable of identifying the six most frequent rearrangements of the T cell receptor (TCR)-δ gene segments in childhood acute lymphoblastic leukemia (ALL). The PCR products amplified in a single reaction are of different size for each TCR-δ gene rearrangement. Therefore, they are readily and unambiguously distinguished after agarose gel electrophoresis and assigned to a specific V-D-J gene rearrangement. There is no need for labor-intensive and time-consuming Southern blot hybridization or nested PCR. To evaluate the multiplex assay we chose 45 DNA samples of childhood ALL analyzed beforehand for TCR-δ gene rearrangements by Southern blot and single PCR of which 30 showed TCR-δ gene rearrangements. The multiplex PCR results corresponded to the Southern blot and single PCR analyses. The described multiplex PCR enables the detection of clonal markers in about 50% of patients in order to monitor minimal residual disease (MRD) in prospective studies with a high turnover of samples.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 12 print issues and online access
$259.00 per year
only $21.58 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Taube, T., Seeger, K., Beyermann, B. et al. Multiplex PCR for simultaneous detection of the most frequent T cell receptor-δ gene rearrangements in childhood ALL. Leukemia 11, 1978–1982 (1997). https://doi.org/10.1038/sj.leu.2400825
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/sj.leu.2400825
Keywords
This article is cited by
-
Very early/early relapses of acute lymphoblastic leukemia show unexpected changes of clonal markers and high heterogeneity in response to initial and relapse treatment
Leukemia (2011)
-
Leukemia-associated genetic aberrations in mesenchymal stem cells of children with acute lymphoblastic leukemia
Journal of Molecular Medicine (2010)
-
Comparison between TaqMan and LightCycler technologies for quantification of minimal residual disease by using immunoglobulin and T-cell receptor genes consensus probes
Leukemia (2003)
-
Potential of LightCycler technology for quantification of minimal residual disease in childhood acute lymphoblastic leukemia
Leukemia (2000)