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Growth Factors and Cytokines

Interleukin-15 + thioredoxin induce DNA synthesis in B-chronic lymphocytic leukemia cells but not in normal B cells

Leukemia volume 11, pages 1298–1304 (1997)Cite this article

Abstract

We have previously shown that Staphylococcus aureus Cowan strain 1 particles (SAC) + thioredoxin (Trx) + IL-2 may induce B-chronic lymphocytic leukemia (B-CLL) cells to proliferate. In this paper we have examined IL-15, which has activities similar to IL-2, for its ability to stimulate B-CLL cells and compared its activity with that of IL-2. We found that B-CLL cells could be induced to DNA synthesis upon treatment with IL-15 + Trx. The presence of Trx was essential for the IL-15-induced DNA synthesis. This contrasts to the effect of IL-15 + Trx on normal CD5+ and CD5− B cells, where IL-15 + Trx alone only induced limited DNA synthesis. IL-15 was as effective in the induction of DNA synthesis in B-CLL cells as IL-2, but about 100-fold less potent with an EC50 of 200 ng/ml. In addition we found that the IL-15 + Trx-induced proliferation was inhibited by CD40 stimulation. We conclude that IL-15 together with a proper costimulus can induce B-CLL cells to proliferate in vitro.

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Authors and Affiliations

  1. Department of Pathology, University of Uppsala, University Hospital, Uppsala, Sweden

    O Söderberg, G Nilsson, M Carlsson & K Nilsson

  2. Department of Clinical Immunology and Transfusion Medicine, University Hospital, Uppsala, Sweden

    I Christiansen

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  1. O Söderberg
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  2. I Christiansen
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  3. G Nilsson
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  4. M Carlsson
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  5. K Nilsson
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Söderberg, O., Christiansen, I., Nilsson, G. et al. Interleukin-15 + thioredoxin induce DNA synthesis in B-chronic lymphocytic leukemia cells but not in normal B cells. Leukemia 11, 1298–1304 (1997). https://doi.org/10.1038/sj.leu.2400728

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  • DOI: https://doi.org/10.1038/sj.leu.2400728

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