Abstract
We performed fluorescence in situ hybridization (FISH) upon 9;22 and 15;17 translocation-positive bone marrow cells to monitor the clinical course of 46 patients with chronic myelocytic leukemia (CML) and nine with acute promyelocytic leukemia (AML M3) who received chemotherapy and/or bone marrow transplantation (BMT). M-BCR-ABL and PML-RARα probes were used to detect translocations of t(9;22) and t(15;17), respectively. Signals from CML patients treated with interferon (17 patients) or BMT (29 patients) were 0.5–15% positive for the 9;22 translocation. Among nine M3 patients who received extensive chemotherapy or BMT, 1–5% were positive for the 15;17 translocation. A highly sensitive FISH procedure using both translocation probes and a whole chromosome Y probe was established and applied to eight sex-mismatched BMT patients (seven CML and one AML M3), in which 0.1–0.6% of signals positive for the specific translocations were detected. These results suggested that interphase FISH is powerful enough to identify minor cell populations of 9;22 or 15;17 translocations after therapy, as well as to detect specific chromosome abnormalities at diagnosis.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 12 print issues and online access
$259.00 per year
only $21.58 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Tanaka, K., Arif, M., Eguchi, M. et al. Application of fluorescence in situ hybridization to detect residual leukemic cells with 9;22 and 15;17 translocations. Leukemia 11, 436–440 (1997). https://doi.org/10.1038/sj.leu.2400583
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/sj.leu.2400583
Keywords
This article is cited by
-
Automated detection of residual cells after sex-mismatched stem-cell transplantation – evidence for presence of disease-marker negative residual cells
Molecular Cytogenetics (2009)
-
Cooperative oncology groups in Japan: Experience from the Japan Adult Leukemia Study Group
Cancer Chemotherapy and Pharmacology (1998)