Abstract
WE have found that a significant percentage of bursectomized irradiated chickens develop a severe anaemia. White leghorn chickens (DeKalb strain 161) were surgically bursectomized when they hatched and given 600 r. of X-irradiation as previously described1. Control animals received X-irradiation only. The animals were housed in conventional animal quarters and given free access to water and food. At 8 weeks of age heparinized blood samples were collected from a wing vein, and red and white blood cells were counted using the Natt–Herrick stain2. Blood smears were prepared and stained with Wright–Giemsa stain, and were examined for the presence of immature erythrocyte forms. Further, the number of lymphocytes per microscopic high power visual field was determined. The haemoglobin concentrations and the haematocrits of the blood samples were determined. The Coombs direct test10 was performed on the blood samples, using a rabbit antiserum raised against chicken IgG as antiglobulin reagent.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Alm, G. V., and Peterson, R. D. A., J. Exp. Med., 129, 1247 (1969).
Natt, M. P., and Herrick, C. A., Poultry Sci., 31, 735 (1952).
Cooper, M. D., Gabrielsen, A. E., and Good, R. A., Ann. Rev. Med., 18, 113 (1967).
Metcalf, D., The Thymus, Recent Results in Cancer Research, 5 (Springer, New York, 1966).
Goodman, J. W., and Shinpock, S. G., Proc. Soc. Exp. Biol. Med., 129, 417 (1968).
Fisher, E. R., in The Thymus in Immunobiology (edit. by Good, R. A., and Gabrielsen, A. E.), 676 (Harper, New York, 1964).
Roland, A. S., Amer. J. Med. Sci., 274, 719 (1964).
Miller, J. F. A. P., Block, M., Rowlands, jun., D. T., and Kind, P., Proc. Soc. Exp. Biol. Med., 118, 916 (1965).
Kellum, M. J., Sutherland, D. E. R., Eckert, E., Peterson, R. D. A., and Good, R. A., Intern. Arch. Allergy Appl. Immunol., 27, 6 (1965).
Levinson, S. A., and MacFate, R. P., Clinical Laboratory Diagnosis, 682 (Lea and Febiger, Philadelphia, 1956).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
ALM, G., PETERSON, R. Anaemia in Bursectomized Irradiated Chickens. Nature 229, 201–202 (1971). https://doi.org/10.1038/229201a0
Received:
Issue Date:
DOI: https://doi.org/10.1038/229201a0
This article is cited by
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
