Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Article
  • Published:

Characterization of the Subunits of Qβ Replicase

Abstract

The RNA replicating enzyme induced by RNA phage Qβ consists of a single phage specific and three host specific polypeptide chains. At least one of the host subunits is shown to be essential for enzymatic activity, and none are known subunits of E. coli DNA-dependent RNA polymerase.

This is a preview of subscription content, access via your institution

Access options

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Weissmann, C., Simon, L., and Ochoa, S., Proc. US Nat. Acad. Sci., 49, 407 (1963).

    Article  ADS  CAS  Google Scholar 

  2. August, J. T., Cooper, S., Shapiro, L., and Zinder, N. D., Cold Spring Harbor Symp. Quant. Biol., 23, 95 (1963).

    Article  Google Scholar 

  3. Haruna, I., Nozu, K., Ohtaka, Y., and Spiegelman, S., Proc. US Nat. Acad. Sci., 50, 905 (1963).

    Article  ADS  CAS  Google Scholar 

  4. Gussin, G. N., J. Mol. Biol., 21, 435 (1966).

    Article  CAS  Google Scholar 

  5. Lodish, H. F., and Zinder, N. D., Science, 152, 372 (1966).

    Article  ADS  CAS  Google Scholar 

  6. Capecchi, M. R., and Gussin, G. N., Science, 149, 417 (1965).

    Article  ADS  CAS  Google Scholar 

  7. Nathans, D., Oeschger, M. P., Eggen, K., and Shimura, Y., Proc. US Nat. Acad. Sci., 56, 1844 (1966).

    Article  ADS  CAS  Google Scholar 

  8. Steitz, J. A., J. Mol. Biol., 33, 923 (1968).

    Article  CAS  Google Scholar 

  9. Haruna, I., and Spiegelman, S., Proc. US Nat. Acad. Sci., 54, 579 (1965).

    Article  ADS  CAS  Google Scholar 

  10. Pace, N. R., Haruna, I., and Spiegelman, S., in Methods in Enzymology (edit. by Grossman, L., and Moldave, K.), 540 (Academic Press, New York, 1968).

    Google Scholar 

  11. Feix, G., Pollet, R., and Weissmann, C., Proc. US Nat. Acad. Sci., 59, 145 (1968).

    Article  ADS  CAS  Google Scholar 

  12. Hori, K., Banerjee, A. K., Eoyang, L., and August, J. T., Proc. US Nat. Acad. Sci., 57, 1790 (1967).

    Article  ADS  CAS  Google Scholar 

  13. Eoyang, L., and August, J. T., in Methods in Enzymology (edit. by Grossman, L., and Moldave, K.), 530 (Academic Press, New York, 1968).

    Google Scholar 

  14. Spiegelman, S., Haruna, I., Holland, I. B., Beaudreau, G., and Mills, D., Proc. US Nat. Acad. Sci., 54, 919 (1965).

    Article  ADS  CAS  Google Scholar 

  15. Weissmann, C., Feix, G., and Slor, H., Cold Spring Harbor Symp. Quant. Biol., 33, 83 (1968).

    Article  CAS  Google Scholar 

  16. Haruna, I., and Spiegelman, S., Proc. US Nat. Acad. Sci., 54, 579 (1965).

    Article  ADS  CAS  Google Scholar 

  17. August, J. T., Banerjee, A. K., Eoyang, L., Franze de Fernandez, M. T., Hori, K., Kuo, C. H., Rensing, U., and Shapiro, L., Cold Spring Harbor Symp. Quant. Biol., 33, 73 (1968).

    Article  CAS  Google Scholar 

  18. Banerjee, A. K., Kuo, C. H., and August, J. T., J. Mol. Biol., 40, 445 (1969).

    Article  CAS  Google Scholar 

  19. Banerjee, A. K., Reusing, U., and August, J. T., J. Mol. Biol., 45 (in the press).

  20. Spiegelman, S., Pace, N. R., Mills, D. R., Levisohn, R., Eikholm, T. S., Taylor, M. M., Peterson, R. L., and Bishop, D. H. L., Cold Spring Harbor Symp. Quant. Biol., 33, 101 (1968).

    Article  CAS  Google Scholar 

  21. Eikholm, T. S., and Spiegelman, S., Proc. US Nat. Acad. Sci., 57, 1833 (1967).

    Article  ADS  Google Scholar 

  22. Shapiro, L., Franze de Fernandez, M. T., and August, J. T., Nature, 220, 478 (1968).

    Article  ADS  CAS  Google Scholar 

  23. Franze de Fernandez, M. T., Eoyang, L., and August, J. T., Nature, 219, 588 (1968).

    Article  ADS  CAS  Google Scholar 

  24. Kondo, M., Gallerani, R., and Weissmann, C., Nature, 228, 525 (1970) (preceding article).

    Article  ADS  CAS  Google Scholar 

  25. diMauro, E., Synder, L., Marino, P., Lamberti, A., Coppo, A., and Tocchini-Valentini, G. P., Nature, 222, 533 (1969).

    Article  ADS  CAS  Google Scholar 

  26. Watanabe, I., Haruna, I., Yamada, Y., Nagaoka, K., and Seki, S., Proc. Jap. Acad., 44, 1038 (1968).

    Article  CAS  Google Scholar 

  27. Bandle, E., and Weissmann, C., Biochim. Biophys. Acta, 199, 551 (1970).

    Article  CAS  Google Scholar 

  28. Babinet, C., Biochem. Biophys. Res. Commun., 26, 639 (1967).

    Article  CAS  Google Scholar 

  29. Burgess, R. R., J. Biol. Chem., 244, 6160 (1969).

    CAS  Google Scholar 

  30. Shapiro, A. L., Vinuela, E., and Maizel, jun., J. V., Biochem. Biophys. Res. Commun., 28, 815 (1967).

    Article  CAS  Google Scholar 

  31. Weber, K., and Osborn, M., J. Biol. Chem., 244, 4406 (1969).

    CAS  Google Scholar 

  32. Burgess, R. R., J. Biol. Chem., 244, 6168 (1969).

    CAS  Google Scholar 

  33. Fairbanks, jun., G., Levinthal, C., and Reeder, R. H., Biochem. Biophys. Res. Commun., 20, 393 (1965).

    Article  CAS  Google Scholar 

  34. Nathans, D., Oeschger, M. P., Eggen, K., and Shimura, Y., Proc. US Nat. Acad. Sci., 56, 1844 (1966).

    Article  ADS  CAS  Google Scholar 

  35. Vinuela, E., Algranati, I. D., and Ochoa, S., Europ. J. Biochem., 1, 3 (1967).

    Article  CAS  Google Scholar 

  36. Nathans, D., Oeschger, M. P., Polmar, S. K., and Eggen, K., J. Mol. Biol., 39, 279 (1969).

    Article  CAS  Google Scholar 

  37. Fromageot, H. P. M., and Zinder, N. D., Proc. US Nat. Acad. Sci., 61, 184 (1968).

    Article  ADS  CAS  Google Scholar 

  38. Vinuela, E., Algranati, I. D., Feix, G., Garwes, D., Weissmann, C., and Ochoa, S., Biochim. Biophys. Acta, 155, 558 (1968).

    Article  CAS  Google Scholar 

  39. Garwes, D., Sillero, A., and Ochoa, S., Biochim. Biophys. Acta, 186, 166 (1969).

    Article  CAS  Google Scholar 

  40. Travers, A. A., and Burgess, R. R., Nature, 222, 537 (1969).

    Article  ADS  CAS  Google Scholar 

  41. Reiner, A. M., J. Bact., 97, 1437 (1969).

    CAS  PubMed  Google Scholar 

  42. Nozawa, R., and Mizuno, D., J. Biochem., 63, 795 (1968).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

KAMEN, R. Characterization of the Subunits of Qβ Replicase. Nature 228, 527–533 (1970). https://doi.org/10.1038/228527a0

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1038/228527a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing