Abstract
WE report here the detection of resonance Raman (RR)1–11 scattering of laser radiation from vibrational modes in the molecules of the carotenoid pigments12–14 lycopene and β-carotene in intact plant samples (Figs. 1a and b). Resonance enhancement is obtained in the Raman spectra of pigments excited at wavelengths in the region of electronic absorption. The effect is manifest in a conspicuous gain in the scattering efficiency from some of the vibrational modes of the pigment. The scattered light becomes sufficiently intense to overcome the increased absorption losses of both the exciting and the scattered radiation. In this study we have demonstrated this effect in heterogeneous (tissue) samples, in which the pigment is just one constituent.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Rent or buy this article
Prices vary by article type
from$1.95
to$39.95
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Harrand, M., CR. Acad. Sci., 226, 241 (1948).
Shorygin, P. P., Dokl. Akad. Nauk SSSR, 87, 201 (1952).
Behringer, J., and Brandmüller, J., Z. Elektrochem., 60, 643 (1956) (review).
Behringer, J., Z. Elektrochem., 62, 544 (1958).
Brandmüller, J., Z. Anal. Chem., 170, 29 (1959).
Ovander, L. N., Opt. i Spektroskopiya, 4, 556 (1958).
Maier, W., and Dörr, F., Appl. Spectrosc., 14, 1 (1960).
Albrecht, A., J. Chem. Phys., 33, 156 (1960).
Jones, A. C., and Tunnicliff, D. D., Anal. Chem., 34, 261R (1962).
Zubov, V. A., in Physical Optics, Proc. Lebedev Phys. Inst., Acad. Sci. USSR (edit. by Skobel'tsyn, D. V.), English translation, 30 (Consultants Bureau, New York, 1966).
Behringer, J., in Raman Spectroscopy (edit. by Szymanski, H. A.), chap. 6 (Plenum, New York, 1967).
Karrer, P., and Jucker, E., Carotenoids (Elsevier, Amsterdam, 1950).
Zechmeister, L., Cis-Trans Isomeric Carotenoids, Vitamins A and Arylpolyenes (Academic Press, New York, and Springer Verlag, Vienna, 1962).
Chemistry and Biochemistry of Plant Pigments (edit. by Goodwin, T. W.) (Academic Press, London and New York, 1965).
Rimai, L., Kilponen, R. G., and Gill, D., J. Amer. Chem. Soc., 92, 3824 (1970).
Behringer, J., Z. Elektrochem., 62, 906 (1958).
Stepanov, B. I., and Apanasevich, P. A., Izv. Akad. Nauk SSSR, Ser. Phys., 22, 1370 (1958).
Shorygin, P. P., and Krushinskii, L. I., Dokl. Akad. Nauk SSSR, 154, 571 (1964).
Behringer, J., and Brandmüller, J., Ann. Physik, 4, 234 (1959).
Ivanova, T. M., Yanovskaya, L. A., and Shorygin, P. P., Opt. i Spektroskopiya, 18, 206 (1965).
Shorygin, P. P., and Ivanova, T. M., Dokl. Akad. Nauk SSSR, 150, 533 (1963).
Shorygin, P. P., and Ivanova, T. M., Opt. i Spektroskopiya, 15, 176 (1963).
Hendra, P. J., and Stratton, P. M., Chem. Rev., 69, 325 (1969).
Weber, G., Biochem. J., 51, 155 (1952).
Stryer, L., Science, 162, 526 (1968).
Griffith, O. H., and Waggoner, A. S., Accounts Chem. Res., 2, 17 (1969).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
GILL, D., KILPONEN, R. & RIMAI, L. Resonance Raman Scattering of Laser Radiation by Vibrational Modes of Carotenoid Pigment Molecules in Intact Plant Tissues. Nature 227, 743–744 (1970). https://doi.org/10.1038/227743a0
Received:
Issue Date:
DOI: https://doi.org/10.1038/227743a0
This article is cited by
-
Isomerization of carotenoids in photosynthesis and metabolic adaptation
Biophysical Reviews (2023)
-
Discovery of carotenoid red-shift in endolithic cyanobacteria from the Atacama Desert
Scientific Reports (2017)
-
Using Raman spectroscopy to characterize biological materials
Nature Protocols (2016)
-
Nondestructive and Rapid Assessment of Intact Tomato Freshness and Lycopene Content Based on a Miniaturized Raman Spectroscopic System and Colorimetry
Food Analytical Methods (2016)
-
Raman imaging in geomicrobiology: endolithic phototrophic microorganisms in gypsum from the extreme sun irradiation area in the Atacama Desert
Analytical and Bioanalytical Chemistry (2016)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.