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Isolation of 4-Chloroindolyl-3-acetic Acid from Immature Seeds of Pisum sativum

Abstract

METHANOL extracts of immature seeds of Pisum sativum exhibited fairly strong auxin-like activity when assayed by hypocotyl swelling of Phaseolus mungo1. We isolated an active principle from the neutral fraction of immature seeds2; it was identified as methyl 4-chloroindolyl-3-acetate by synthesis. Preliminary examination showed that its auxin activity against Avena first internode and section tests was comparable with that of methyl indolyl-3-acetate while its activity was stronger against the hypocotyl swelling test of Phaseolus mungo. Gandar and Nitsch3 independently isolated the same auxin from the same immature seeds, and found it to be a methyl ester of chlorine containing indolyl-3-acetic acid.

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References

  1. Key, J. L., Hanson, J. B., and Bills, R. F., Plant Physiol., 35, 177 (1966).

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  2. Marumo, S., Abe, H., Hattori, H., and Munakata, K., Agric. Biol. Chem. (Japan), 32(1), 117 (1968).

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  3. Gandar, J. C., and Nitsch, C., CR Acad. Sci. Paris, 265, 1795 (1967).

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MARUMO, S., HATTORI, H., ABE, H. et al. Isolation of 4-Chloroindolyl-3-acetic Acid from Immature Seeds of Pisum sativum. Nature 219, 959–960 (1968). https://doi.org/10.1038/219959b0

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