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Dissociation and Inhibition of the Stimulatory Effect of Phytohaemagglutinin on Protein and DNA Synthesis in Human Lymphocyte Cultures

Nature volume 214, pages 1240–1241 (1967)Cite this article

Abstract

PHYTOHAEMAGGLUTININ (PHA) stimulates both DNA1 and protein synthesis2 by human peripheral white blood cells in tissue culture. Evidence to be presented indicates that this phenomenon is partly dependent on serum factors. The maximum synthesis of these two macromolecules in stimulated cultures occurs at different concentrations of serum in the tissue culture medium. In addition, increases in the concentration of serum beyond that which provided for maximal synthesis resulted in inhibition of the PHA stimulation on both DNA and protein synthesis. Variation in the serum content of unstimulated cultures had no significant effect on the synthesis of these substances. These results were independent of the source of serum, that is, whether autologous, homologoiis or heterologous.

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References

  1. McIntyre, O. R., and Ebaugh, jun., F. G., Blood, 19, 443 (1962).

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Authors and Affiliations

  1. V and VI Medical Service, Boston City Hospital,

    S. R. COOPERBAND, J. A. GREEN, M. A. KENNEDY & M. M. GRANT

  2. Departments of Medicine and Microbiology, Boston University Medical School, Boston, Massachusetts

    S. R. COOPERBAND, J. A. GREEN, M. A. KENNEDY & M. M. GRANT

Authors
  1. S. R. COOPERBAND
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  2. J. A. GREEN
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  3. M. A. KENNEDY
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  4. M. M. GRANT
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COOPERBAND, S., GREEN, J., KENNEDY, M. et al. Dissociation and Inhibition of the Stimulatory Effect of Phytohaemagglutinin on Protein and DNA Synthesis in Human Lymphocyte Cultures. Nature 214, 1240–1241 (1967). https://doi.org/10.1038/2141240a0

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  • DOI: https://doi.org/10.1038/2141240a0

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