Abstract
It would be a considerable help in investigations of the nervous system if it were possible to isolate neurones in large numbers with reasonably intact processes and completely free from contamination. Methods for the separation of neuronal or glial cells have been based either on the microdissection techniques of Lowry1 and Hydén2, or on the dispersion of nervous tissue and subsequent isolation of cells by hand collection3 or differential centrifugation4. Hand dissection enables rapid isolation of a few selected nerve cells the biochemical and physiological properties of which appear to be normal5,6, whereas the mechanical process is lengthy and possibly too drastic.
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BOCCI, V. Enzyme and Metabolic Properties of Isolated Neurones. Nature 212, 826–827 (1966). https://doi.org/10.1038/212826a0
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DOI: https://doi.org/10.1038/212826a0
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