Abstract
THE chemistry of the Sakaguchi reaction—between arginine, l-naphthol and alkaline hypochlorite—is not known. As originally described 40 years ago1, this sensitive reaction fortuitously revealed itself as a red coloration which slowly developed and faded. By substituting hypobromite2 for hypochlorite, almost instantaneous colour development was effected, and more recently Szilagyi and Szabo3 achieved an improvement in the reproducibility of the reaction with the aid of N-bromosuccinimide (BSI)—a more stable source of available bromine.
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WATSON, D. Chromogenicity of Sakaguchi-reactive Compounds, Monosubstituted Guanidines and Histidine. Nature 211, 411–412 (1966). https://doi.org/10.1038/211411a0
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DOI: https://doi.org/10.1038/211411a0
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