Abstract
ENRICHMENT methods have greatly facilitated the work of geneticists in obtaining desired mutants. The various methods in use depend on the selective removal or killing of growing cells relative to non-growing mutants. Moat et al.1 showed that several antifungal agents could be used with yeast to increase the proportion of mutant survivors in artificial mixtures of mutant and wild-type cells. Although ‘Nystatin’ was not so effective as ‘Endomycin’ or ‘Amphotericin B’ in their experiments, it seemed a desirable antibiotic with which to undertake further work, as a great deal is known about the physiology of its action on yeast2–4 and especially as it had been reported that actively metabolizing cells take up much more of it than glucose-starved cells5.
Access options
Subscribe to Journal
Get full journal access for 1 year
220,50 €
only 4,32 € per issue
All prices include VAT for France.
Rent or Buy article
Get time limited or full article access on ReadCube.
from$8.99
All prices are NET prices.
References
- 1.
Moat, A. C., Peters, N., and Srb, A. M., J. Bact., 77, 673 (1959).
- 2.
Lampen, J. O., Arnow, P. M., and Safferman, R. S., J. Bact., 80, 200 (1960).
- 3.
Marini, F., Arnow, P., and Lampen, J. O., J. Gen. Microbiol., 24, 51 (1961).
- 4.
Lampen, J. O., Arnow, P. M., Borowska, Z., and Laskin, A. I., J. Bact., 84, 1152 (1962).
- 5.
Lampen, J. O., Morgan, E. R., Slocum, A., and Arnow, P., J. Bact., 78, 282 (1959).
- 6.
Gorini, L., and Kaufman, H., Science, 131, 604 (1960).
- 7.
Lederberg, J., and Zinder, N., J. Amer. Chem. Soc., 70, 4267 (1948).
- 8.
Davis, B. D., Proc. U.S. Nat. Acad. Sci., 35, 1 (1949).
Author information
Author notes
- RICHARD SNOW
Permanent address: Department of Genetics, University of California, Davis, California.
Affiliations
Department of Genetics, John Innes Institute, Bayfordbury, Hertford, Hertfordshire.
- RICHARD SNOW
Authors
Search for RICHARD SNOW in:
Rights and permissions
To obtain permission to re-use content from this article visit RightsLink.
About this article
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
