Abstract
INTRACISTRON mapping in maize has been demonstrated by Nelson1,2 with a technique based on differential staining reactions of starch in pollen of different genotypes. Those with the waxy (wx) locus stain reddish brown in iodine–potassium iodide solutions; whereas non-waxy (Wx) pollen stains dark blue. Therefore, in waxy plants genetic recombination or back-mutation at this locus is manifested by the appearance of dark blue staining pollen. The technique has a distinct advantage in genetic investigations with higher plants in that large populations of hundreds of thousands of genotypes (pollen grains) can be scored with ease.
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References
Nelson, Oliver E., Science, 130, 794 (1959).
Nelson, Oliver E., Genetics, 47, 737 (1962).
Amano, E., and Smith, H. H., Mutation Research (in the press).
Briggs, R. W., and Smith, H. H., J. Heredity (in the press).
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BRIGGS, R., AMANO, E. & SMITH, H. Genetic Recombination with Ethyl-methanesulphonate-induced Waxy Mutants in-Maize. Nature 207, 890–891 (1965). https://doi.org/10.1038/207890a0
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DOI: https://doi.org/10.1038/207890a0
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